TOP2B Antibody

Images

 
Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta ...read more
Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Independent Antibodies: Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit ...read more
Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TOP2B Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TOP2B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Immunoprecipitation 2-10 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Theoretical MW
183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
TOP2B Knockout 293T Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-40842 in the following applications:

Publications
Read Publications using
NB100-40842 in the following applications:

  • 1 publication
  • IP
    1 publication
  • WB
    2 publications

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24270455).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TOP2B Antibody

  • antigen MLAA-44
  • DNA topoisomerase 2-beta
  • DNA topoisomerase II beta
  • DNA topoisomerase II, 180 kD
  • DNA topoisomerase II, beta isozyme
  • EC 5.99.1.3
  • TOP2B
  • top2beta
  • TOPIIB
  • topo II beta
  • topoisomerase (DNA) II beta (180kD)
  • topoisomerase (DNA) II beta 180kDa
  • topoisomerase II beta
  • topoisomerase IIb
  • U937 associated antigen

Background

Topo II Beta antibody plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Further, it is implicated in drug resistance of tumor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-40842
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP

Publications for TOP2B Antibody (NB100-40842)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: ChIP, IP, WB.


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(1)
IP
(1)
WB
(2)
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Mouse
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Showing Publications 1 - 7 of 7.
Publications using NB100-40842 Applications Species
Alvarez-QuilOn A, TerrOn-Bautista J, Delgado-Sainz I et al. Endogenous topoisomerase II-mediated DNA breaks drive thymic cancer predisposition linked to ATM deficiency Nat Commun Feb 14 2020 [PMID: 32060399] (IP, Mouse) IP Mouse
Sciascia N, Wu W, Zong D et Al. Suppressing proteasome mediated processing of topoisomerase II DNA-protein complexes preserves genome integrity Elife Feb 14 2020 [PMID: 32057297] (WB, Mouse) WB Mouse
Hedayati M, Haffner MC, Coulter JB et al. Androgen Deprivation Followed by Acute Androgen Stimulation Selectively Sensitizes AR-Positive Prostate Cancer Cells to Ionizing Radiation. Clin Cancer Res 2016 Jul 1 [PMID: 26831716]
Maxwell SS, Pelka GJ, Tam PP, El-Osta A. Chromatin context and ncRNA highlight targets of MeCP2 in brain. RNA Biol 2013 Nov [PMID: 24270455] (Mouse) Mouse
Elliott SL, Crawford C, Mulligan E et al. Mitoxantrone in combination with an inhibitor of DNA-dependent protein kinase: a potential therapy for high risk B-cell chronic lymphocytic leukaemia. Br J Haematol 2011 Jan [PMID: 21083655]
Cortes-Ledesma F, Martinez-Garcia Pm Genome-wide prediction of topoisomerase II beta binding by architectural factors and chromatin accessibility bioRxiv Jan 1 2020 (ChIP, Human, Mouse) ChIP Human, Mouse
Chatzinikolaou G, Stratigi K, Agathangelou K ERCC1-XPF Interacts with Topoisomerase II beta to Facilitate the Repair of Activity-induced DNA Breaks bioRxiv Jan 1 2020 (WB, Mouse) WB Mouse

Review for TOP2B Antibody (NB100-40842) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-40842:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot TOP2B NB100-40842
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4
reviewed by:
Anonymous
WB Human 08/24/2015
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Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

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Recent Reviews

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Anonymous
08/24/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol TOP2B
Entrez
Uniprot