Western Blot: TOP2B Antibody [NB100-40842] - Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta ...read more
Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbitanti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
Independent Antibodies: Western Blot: TOP2B Antibody [NB100-40842] - Detection of Human Topo II Beta on HeLa whole cell lysate using NB100-40842. Topo II Beta was also immunoprecipitated by another rabbit ...read more
Immunohistochemistry-Paraffin: TOP2B Antibody [NB100-40842] - Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB
The immunogen recognized by this antibody maps to a region between residue 1570 and the C-terminus (residue 1621) of human DNA Topoisomerase II Beta using the numbering given in entry NP_001059.2 (GeneID 7155).
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
183 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in scientific literature (PMID: 24270455).
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
TBS and 0.1% BSA
0.09% Sodium Azide
Immunogen affinity purified
Alternate Names for TOP2B Antibody
DNA topoisomerase 2-beta
DNA topoisomerase II beta
DNA topoisomerase II, 180 kD
DNA topoisomerase II, beta isozyme
topo II beta
topoisomerase (DNA) II beta (180kD)
topoisomerase (DNA) II beta 180kDa
topoisomerase II beta
U937 associated antigen
Topo II Beta antibody plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Further, it is implicated in drug resistance of tumor cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.