TNFRH3/TNFRSF26 Antibody [Unconjugated]

Western blot shows lysates of mouse thymus tissue and mouse spleen tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5330) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TNFRH3/TNFRSF26 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Mouse splenocytes were stained with Sheep Anti-Mouse TNFRH3/TNFRSF26 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF5330, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 637-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL011).

• Reactivity: Mu
• Applications: WB, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TNFRH3/TNFRSF26 Antibody [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse TNFRH3/TNFRSF26
  Ser20-Lys162
  Accession # P83626
• Specificity: Detects mouse TNFRH3/TNFRSF26 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant mouse (rm) TNF RI and rmTNF RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: Tnfrsf26
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNFRH3/TNFRSF26 Antibody [Unconjugated]

• APO-3;DDR3;DR3;GEF720;LARD;PLEKHG5;TNFRSF12;TNFRSF25;TR3;TRAMP;Tumor necrosis factor receptor superfamily member 25;WSL-1;WSL-LR
• TNFRH3
• Tnfrsf24
• Tnfrsf26

Background

TNFRH3 (Tumor necrosis factor receptor homolog 3; also TNFR superfamily member 26) is a 21 kDa (predicted) member of the TNF receptor superfamily. It appears to be concentrated in lymphoid cell types. Mature mouse TNFRH3 is a type I transmembrane protein that is 185 amino acid (aa) in length. It contains a 145 aa extracellular region and a 19 aa cytoplasmic domain. The extracellular region contains three TNFR-Cys repeats that characterize TNF receptors (aa 27‑143). Unlike two related TNF receptor homologs, TNFRH3 does not serve as a TRAIL decoy receptor. No ortholog to mouse TNFRH3 has been reported in human or rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Tnfrsf26
• Uniprot:
  • P83626()