TNF RII/TNFRSF1B Antibody [Biotin]

• Reactivity: Hu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

TNF RII/TNFRSF1B Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human sTNF RII/TNFRSF1B
• Specificity: Detects human TNF RII/TNFRSF1B in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human TNF RI, recombinant mouse (rm) TNF RI, and rmTNF RII is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TNFRSF1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human TNF RII/TNFRSF1B Antibody (Catalog # MAB726)
  ELISA Detection: Human TNF RII/TNFRSF1B Biotinylated Antibody (Catalog # BAF726)
  Standard: Recombinant Human TNF RII/TNFRSF1B aa 24-206 (Catalog # 1089-R2)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TNF RII/TNFRSF1B Antibody [Biotin]

• CD120b antigen
• CD120b
• Etanercept
• p75 TNF receptor
• p75TBPII
• p75TNFR
• soluble TNFR1B variant 1
• TNF RII
• TNFBRp80 TNF-alpha receptor
• TNF-R2
• TNFR2TNFR1B
• TNF-R75
• TNFR80
• TNFRII
• TNF-RII
• TNF-R-II
• TNFR-II
• TNFRSF1B
• tumor necrosis factor beta receptor
• tumor necrosis factor binding protein 2
• Tumor necrosis factor receptor 2
• tumor necrosis factor receptor superfamily member 1B
• tumor necrosis factor receptor superfamily, member 1B
• Tumor necrosis factor receptor type II

Background

TNF RII, also known as p75 or p80 TNF R, is a prototypic member of the TNF receptor superfamily and has been designated TNFRSF1B. It binds TNF with high affinity and is expressed primarily in cells of the immune system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TNF RII/TNFRSF1B Antibody (BAF726)(6)

Publications using BAF726

• T Baram, N Oren, N Erlichman, T Meshel, A Ben-Baruch Inflammation-Driven Regulation of PD-L1 and PD-L2, and Their Cross-Interactions with Protective Soluble TNFalpha Receptors in Human Triple-Negative Breast Cancer Cancers, 2022-07-19;14(14):. 2022-07-19 [PMID: 35884574] (ELISA Capture, Human)
• Inoue M, Kamada H, Abe Y, Higashisaka K, Nagano K, Mukai Y, Yoshioka Y, Tsutsumi Y, Tsunoda S Aminopeptidase P3, a new member of the TNF-TNFR2 signaling complex, induces phosphorylation of JNK1 and JNK2. J Cell Sci, 2015-01-20;128(4):656-69. 2015-01-20 [PMID: 25609706] (Western Blot, Human)
• Hu H, Kwun J, Aizenstein BD, Knechtle SJ Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine. Transplantation, 2009-06-27;87(12):1814-20. 2009-06-27 [PMID: 19543058] (Antibody Array Development, Human)
• Copeland S, Siddiqui J, Remick D Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines. J. Immunol. Methods, 2004-01-01;284(1):99-106. 2004-01-01 [PMID: 14736420] (Array Development, Human)
• Jyonouchi H, Sun S, Itokazu N Innate immunity associated with inflammatory responses and cytokine production against common dietary proteins in patients with autism spectrum disorder. Neuropsychobiology, 2002-01-01;46(2):76-84. 2002-01-01 [PMID: 12378124] (ELISA Development, Human)
• Bharucha AE, 2019, Jorgensen R, Lichtman SN, LaRusso NF, Lindor KD A pilot study of pentoxifylline for the treatment of primary sclerosing cholangitis. 2000-09-01;95(9):2338-42. 2000-09-01 [PMID: 11007239] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: TNFRSF1B