Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Sandwich ELISA: Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP1-92722] - Representative standard curve for monkey TNF-R I ELISA. Monkey TNF-R I was diluted in serial 2-fold steps in Assay Buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.

Product Discontinued

Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric) Summary

• Description: The monkey sTNF-R I ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of monkey sTNF-R I
• Specificity: The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a monkey TNF-R (60 kDa) positive serum. There was no crossreactivity detected.
• Standard Curve Range: 0.09 - 6.0 U/mL
• Sensitivity: 0.06 U/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 9.0%
• Intra-Assay: CV% < 2.0%
• Spike Recovery: The overall mean recovery was 93%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF1A

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Monkey TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor. Germline mutations of the extracellular domains of this receptor were found to be associated with the autosomal dominant periodic fever syndrome. The impaired receptor clearance is thought to be a mechanism of the disease. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TNF RI/TNFRSF1A ELISA Kit (NBP1-92722). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFRSF1A