Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Sandwich ELISA: Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP1-83738] - Representative standard curve for human TNF-R (60 kDa) ELISA. Human TNF-R (60 kDa) was diluted in serial 2-fold steps in Assay buffer (1x). Do not use this standard curve to derive test results. A standard curve must be run foreach group of microwell strips assayed.

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), urine
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.05 ng/mL

Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) Summary

• Description: The human sTNF-R (60 kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sTNF-R (60 kDa)
• Specificity: The cross reactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF RI/TNFRSF1A (60 kDa) positive serum (50% calf serum containing 5 ng/ml TNF RI/TNFRSF1A (60 kDa)). There was no cross reactivity or interference detected, notably not with human TNF alpha (< 10 ng/ml) and TNF beta (< 100 ug/ml)
• Standard Curve Range: 0.08 - 5.0 ng/mL
• Sensitivity: 0.05 ng/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 8.6%
• Intra-Assay: CV% < 1.9%
• Spike Recovery: The recovery ranged from 91% to 96% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 10 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFRSF1A

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

• CD120a antigen
• CD120a
• FPF
• p55
• p55-R
• p60
• TNF RI
• TNFARMGC19588
• TNF-R
• TNF-R1
• TNFR1TBP1
• TNFR55
• TNF-R55
• TNFR60
• TNFRI
• TNF-RI
• TNF-R-I
• TNFR-I
• TNFRSF1A
• tumor necrosis factor binding protein 1
• Tumor necrosis factor receptor 1
• tumor necrosis factor receptor 1A isoform beta
• tumor necrosis factor receptor superfamily member 1A
• tumor necrosis factor receptor superfamily, member 1A
• tumor necrosis factor receptor type 1
• Tumor necrosis factor receptor type I
• tumor necrosis factor-alpha receptor

Background

TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75-80 and 55-60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The exact mechanism of signal transduction after binding of TNF to the receptor is still unclear. The extracellular fragment of the 60 kDa TNF receptor, with a molecular mass of about 30 kDa was purified, partially sequenced, and the respective cDNA was cloned. This TNF binding protein is liberated from the intact molecule by proteolytic cleavage and comprises most of the extracellular portion of the receptor, including all three N-glycosylation sites. The present assay provides a simple, rapid, and highly sensitive method for the determination of soluble TNF-R (60 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF1A