Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

Images

 
Sandwich ELISA: Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) [NBP1-83738] - Representative standard curve for human TNF-R (60 kDa) ELISA. Human TNF-R (60 kDa) was diluted in serial 2-fold steps in Assay buffer (1x). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Standard Curve Range
0.08 - 5.0 ng/mL
Sensitivity
0.05 ng/mL

Order Details

Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) Summary

Specificity
The cross reactivity and interference of circulating factors of the immune system were evaluated by spiking these proteins at physiologically relevant concentrations into a human TNF RI/TNFRSF1A (60 kDa) positive serum (50% calf serum containing 5 ng/ml TNF RI/TNFRSF1A (60 kDa)). There was no cross reactivity or interference detected, notably not with human TNF alpha (< 10 ng/ml) and TNF beta (< 100 ug/ml)
Standard Curve Range
0.08 - 5.0 ng/mL
Sensitivity
0.05 ng/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.6%
Intra-Assay
1.9%
Spike Recovery
The recovery ranged from 91% to 96% with an overall mean recovery of 93%. Please see protocol for more specific Spike Recovery details.
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TNFRSF1A

Applications/Dilutions

Application Notes
The human sTNF-R (60 kDa) ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sTNF-R (60 kDa)

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Adhesive Films
  2. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Adiponectin
  3. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
  4. Biotin-Conjugate anti-human Adiponectin monoclonal antibody
  5. Human Adiponectin Standard lyophilized, 30 ng/ml upon reconstitution
  6. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  7. Streptavidin-HRP
  8. Substrate Solution (tetramethyl-benzidine)
  9. Stop Solution (1M Phosphoric acid)

Alternate Names for Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric)

  • CD120a antigen
  • CD120a
  • FPF
  • p55
  • p55-R
  • p60
  • TNF RI
  • TNFARMGC19588
  • TNF-R
  • TNF-R1
  • TNFR1TBP1
  • TNFR55
  • TNF-R55
  • TNFR60
  • TNFRI
  • TNF-RI
  • TNF-R-I
  • TNFR-I
  • TNFRSF1A
  • tumor necrosis factor binding protein 1
  • Tumor necrosis factor receptor 1
  • tumor necrosis factor receptor 1A isoform beta
  • tumor necrosis factor receptor superfamily member 1A
  • tumor necrosis factor receptor superfamily, member 1A
  • tumor necrosis factor receptor type 1
  • Tumor necrosis factor receptor type I
  • tumor necrosis factor-alpha receptor

Background

TNF-receptors have been demonstrated on a wide variety of human somatic cells including fibroblasts, endothelial cells, adipocytes, liver membranes, granulocytes and several tumor cell lines. Based on gel filtration experiments the receptor appears to be a complex of different proteins with a molecular weight of 350 kDa. In a variety of cell lines two different types of TNF receptors with 75-80 and 55-60 kDa respectively have been identified. The cDNAs encoding the two different TNF receptors have been cloned. The exact mechanism of signal transduction after binding of TNF to the receptor is still unclear. The extracellular fragment of the 60 kDa TNF receptor, with a molecular mass of about 30 kDa was purified, partially sequenced, and the respective cDNA was cloned. This TNF binding protein is liberated from the intact molecule by proteolytic cleavage and comprises most of the extracellular portion of the receptor, including all three N-glycosylation sites. The present assay provides a simple, rapid, and highly sensitive method for the determination of soluble TNF-R (60 kDa) levels in body fluids or cell culture supernatants. This assay will help to clarify the possible diagnostic and prognostic value of circulating sTNF-R (60 kDa) in various neoplastic and inflammatory diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
H00003117-M01
Species: Hu
Applications: WB, ELISA, PLA
NBP2-13883
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
MAB224
Species: Hu
Applications: Flow, IHC, CyTOF-ready, Neut
NB110-40561
Species: Hu
Applications: WB, IP
AF5789
Species: Hu, Mu, Rt
Applications: WB
NBP1-18910
Species: Hu, Mu, Rt
Applications: WB, Simple Western, ICC/IF, IHC, IHC-Fr, IHC-P, IP
AF1138
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56715
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-83738
Species: Hu
Applications: ELISA

Publications for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738) (0)

There are no publications for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738) (0)

There are no reviews for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TNF RI/TNFRSF1A Products

Bioinformatics Tool for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738)

Discover related pathways, diseases and genes to TNF RI/TNFRSF1A ELISA Kit (NBP1-83738). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738)

Discover more about diseases related to TNF RI/TNFRSF1A ELISA Kit (NBP1-83738).
 

Pathways for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738)

View related products by pathway.

PTMs for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738)

Learn more about PTMs related to TNF RI/TNFRSF1A ELISA Kit (NBP1-83738).
 

Research Areas for TNF RI/TNFRSF1A ELISA Kit (NBP1-83738)

Find related products by research area.

Blogs on TNF RI/TNFRSF1A

There are no specific blogs for TNF RI/TNFRSF1A, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human TNF RI/TNFRSF1A ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFRSF1A
Entrez