TNF-alpha Antibody (V1q) Summary
Cytotoxin purified from conA induced T cell clone 29
Protein A or G purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
- Blocking/Neutralizing 50-200 molar excess
- Flow Cytometry 1:10-1:1000
Mouse and chinese hamster. Does not react with receptor-bound mouse TNF alpha.
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
PBS, containing 0.1% BSA
Protein A or G purified
Alternate Names for TNF-alpha Antibody (V1q)
- APC1 protein
- TNF, monocyte-derived
- TNFATNF, macrophage-derived
- TNFSF2TNF superfamily, member 2
- tumor necrosis factor (TNF superfamily, member 2)
- tumor necrosis factor alpha
- Tumor necrosis factor ligand superfamily member 2
- tumor necrosis factor
- tumor necrosis factor-alpha
The monoclonal antibody V1q recognizes Tumor Necrosis Factor alpha. Tumor necrosis factor-alpha, a homotrimeric 17 KD protein, is a potent mediator of inflammatory and metabolic functions. TNF-alpha was originally detected as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Further, TNF-alpha has been implied as central mediator in shock induced by gram negative micro-organisms. The cytokine TNF-alpha is found to be a central mediator in many inflammatory and immunological processes: it can be induced by various products of micro-organisms and by various cytokines. It also induces on its turn the production of many cytokines. Furthermore, TNF-alpha has also been found in inflammatory foci such as synovial effusions in rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. Signal transduction occurs via two types of TNF-receptors, the TNF-receptors 1 and 2. The receptors differ strongly in their intra-cellular signaling pathways. The TNF-alpha trimer interacts with either of the two types of TNF-R leading to receptor cross-linking. The monoclonal antibody V1q reacts with both natural and recombinant TNF-alpha and shows neutralizing activity. However, monoclonal antibody V1q does not recognize membrane bound and receptor bound TNF-alpha.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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Product General Protocols
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FAQs for TNF-alpha Antibody (NBP1-51500). (Showing 1 - 4 of 4 FAQ).
I need to know about TNF-alpha antibody kits for mice.
- We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
- I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
- The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
- I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
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