TNF-alpha Antibody (SPM543) [Alexa Fluor (R) 700]


There are currently no images for TNF-alpha Antibody (NBP2-34419AF700).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity Hu, Mu, Rt, Ca, Fe, Rb, ZeSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P, IP
Please see the vial label for concentration. If unlisted please contact technical services.

TNF-alpha Antibody (SPM543) [Alexa Fluor (R) 700] Summary

A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin
Cytoplasmic and extracellular (secreted)
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
IgG1 Kappa
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C in the dark.
Alexa Fluor (R) 700
Alexa Fluor (R) 700
50mM Sodium Borate
0.05% Sodium Azide
Please see the vial label for concentration. If unlisted please contact technical services.
Protein A purified


Application Notes
Immunohistochemistry (Staining of paraffin tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
17 kDa

Alternate Names for TNF-alpha Antibody (SPM543) [Alexa Fluor (R) 700]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
Applications: Func-Inh
Species: Hu
Applications: WB, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu, Mu, Rt, Po, Av, Fe, Fi, Rb, Xp
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, Neut
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
Species: Hu, Mu, Rt, Ca, Fe, Rb, Ze
Applications: Flow, ICC/IF, IHC-Fr, IHC-P, IP

Publications for TNF-alpha Antibody (NBP2-34419AF700) (0)

There are no publications for TNF-alpha Antibody (NBP2-34419AF700).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF-alpha Antibody (NBP2-34419AF700) (0)

There are no reviews for TNF-alpha Antibody (NBP2-34419AF700). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Video Protocols

ICC/IF Video Protocol

FAQs for TNF-alpha Antibody (NBP2-34419AF700). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies


Isotype Controls

Additional TNF-alpha Products

Related Products by Gene

Bioinformatics Tool for TNF-alpha Antibody (NBP2-34419AF700)

Discover related pathways, diseases and genes to TNF-alpha Antibody (NBP2-34419AF700). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF-alpha Antibody (NBP2-34419AF700)

Discover more about diseases related to TNF-alpha Antibody (NBP2-34419AF700).

Pathways for TNF-alpha Antibody (NBP2-34419AF700)

View related products by pathway.

PTMs for TNF-alpha Antibody (NBP2-34419AF700)

Learn more about PTMs related to TNF-alpha Antibody (NBP2-34419AF700).

Research Areas for TNF-alpha Antibody (NBP2-34419AF700)

Find related products by research area.

Blogs on TNF-alpha.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol TNF

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBP2-34419AF700 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

Human TNF-alpha Protein