TNF-alpha Antibody Pair

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Sandwich ELISA: TNF alpha Antibody Pair [H00007124-AP41] - Detection sensitivity ranging from 1 ng/ml to 100 ng/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications S-ELISA
Clonality
Monoclonal
Standard Curve Range
1 ng/ml to 100 ng/ml

Order Details

TNF-alpha Antibody Pair Summary

Description
This antibody pair set comes with matched antibody pair to detect and quantify protein level of human TNF.

  • Capture antibody: mouse monoclonal anti-TNF IgG1 Kappa (100 ug)
  • Detection antibody: biotinylated mouse monoclonal anti-TNF IgG1 Kappa (50 ug)
  • Standard Curve Range
    1 ng/ml to 100 ng/ml
    Assay Type
    Sandwich ELISA
    Clonality
    Monoclonal
    Gene
    TNF
    Purity
    Immunogen affinity purified

    Applications/Dilutions

    Dilutions
    • Sandwich ELISA 0.3 ng/ml - 100 ng/ml

    Packaging, Storage & Formulations

    Storage
    Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
    Preservative
    No Preservative
    Purity
    Immunogen affinity purified

    Notes

    Quality control test: Standard curve using recombinant protein (H00007124-P01) as an analyte.

    This product is produced by and distributed for Abnova, a company based in Taiwan.

    Alternate Names for TNF-alpha Antibody Pair

    • APC1 protein
    • Cachectin
    • Cachetin
    • DIF
    • TNF
    • TNF, monocyte-derived
    • tnfa
    • tnf-a
    • TNFalpha
    • TNF-alpha
    • TNF-alphacachectin
    • TNFATNF, macrophage-derived
    • TNFSF1A
    • TNFSF2
    • TNFSF2TNF superfamily, member 2
    • tumor necrosis factor (TNF superfamily, member 2)
    • tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    • tumor necrosis factor
    • tumor necrosis factor-alpha

    Limitations

    This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

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    FAQs for TNF-alpha Antibody Pair (H00007124-AP41). (Showing 1 - 4 of 4 FAQ).

    1. I need to know about TNF-alpha antibody kits for mice.
      • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
    2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
      • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
    3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
      • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
    4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
      • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

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    Bioinformatics

    Gene Symbol TNF
    Entrez