TNF-alpha Antibody (610325) [Alexa Fluor® 532]

• Applications: WB
• Clone: 610325
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

TNF-alpha Antibody (610325) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant rabbit TNF-α
  Leu79-Leu234
  Accession # P04924
• Specificity: Detects rabbit TNF-alpha in direct ELISAs. In direct ELISAs, 50-100% cross-reactivity with recombinant cotton rat, feline, guinea pig, human, mouse, rat TNF-alpha , less than 10% cross-reactivity with recombinant canine, equine, rhesus TNF-alpha , and no cross-reacti
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Tumor necrosis factor (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily and family. The 26 kDa type II transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Rabbit TNF-alpha is 235 amino acids (aa) in length and contains a 35 aa cytoplasmic domain, a 21 aa transmembrane region, and a 179 extracellular domain (ECD).Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha . Rabbit TNF-alpha is 79% and 78% aa identical to human and mouse TNF-alpha , respectively. TNF-alpha is produced by several lymphoid cells as well as by astrocytes, endothelial cells, and smooth muscle cells. TNF-alpha binds TNF RI and TNF RII present on virtually all cell types where it triggers the activation of multiple signal transduction pathways and modulates the expression of a wide variety of genes.   

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TNF-alpha Antibody (FAB56701X). (Showing 1 - 4 of 4 FAQ).

1. I need to know about TNF-alpha antibody kits for mice.
   • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
   • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
   • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
   • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.