TNF-alpha Antibody (52B83) [FITC]

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Product Details

Summary
Reactivity Hu, Rb, ShSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
52B83
Clonality
Monoclonal
Host
Mouse
Conjugate
FITC

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TNF-alpha Antibody (52B83) [FITC] Summary

Immunogen
The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha).
Localization
Type 2 membrane protein or soluble form derived from the membrane by proteolytic cleavage.
Specificity
This detects both natural and recombinant TNFa. It does not cross-react with TNF beta or lymphotoxin. Non-specific background staining is obesrved in connective tissues.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNF
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Reviewed Applications
Read 1 Review rated 5
using
NBP1-51502 in the following applications:

Publications
Read Publication using
NBP1-51502 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 28114570). Expected cross reactivity baes on sequence identity: Guinea pig, Rhesus Monkey, Mouse. Sheep data from customer review.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.2 um filtered antibody solution in PBS and 1.0% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for TNF-alpha Antibody (52B83) [FITC]

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • tnfa
  • tnf-a
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFSF1A
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha

Background

The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TNF-alpha Antibody (NBP1-51502)(1)

We have publications tested in 1 confirmed species: Rabbit.

We have publications tested in 1 application: IHC.


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IHC
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Review for TNF-alpha Antibody (NBP1-51502) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Rabbit and Mouse.
We have 1 review tested in 1 application: IHC.

Reviews using NBP1-51502:
Filter by Applications
IHC
(1)
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Filter by Species
Rabbit and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC Rabbit and Mouse 04/21/2017
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Summary

ApplicationImmunohistochemistry
Sample Testedmouse cryosections,mouse eye,rabbit eye
SpeciesRabbit and Mouse

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TNF-alpha Antibody (NBP1-51502). (Showing 1 - 4 of 4 FAQ).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TNF-alpha Antibody (NBP1-51502)

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Diseases for TNF-alpha Antibody (NBP1-51502)

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Blogs on TNF-alpha.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

 
Anonymous
04/21/2017
Application: IHC
Species: Rabbit and Mouse

Bioinformatics

Gene Symbol TNF
Entrez