TMEM97 Antibody - BSA Free

Western Blot: TMEM97 Antibody [NBP1-92522] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-92522] - Staining of human pancreas shows moderate to strong cytoplasmic positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-92522] - Staining of human stomach shows moderate cytoplasmic positivity in a subset of glandular cells.

Immunohistochemistry-Paraffin: TMEM97 Antibody [NBP1-92522] - Staining of human testis shows moderate cytoplasmic positivity in Leydig cells.

Staining of human tonsil shows no positivity in lymphoid cells as expected.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TMEM97 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TMEM97 Antibody - BSA Free (NBP1-92522) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: FEDFSKASGFKGQRPETLHERLT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM97
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  TMEM97 Protein (NBP1-92522PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TMEM97 Antibody - BSA Free

• MAC30
• MAC30Protein MAC30
• S2R
• Sigma2 receptor
• Sigma-2 receptor
• TMEM97
• transmembrane protein 97

Background

TMEM97, or MAC30, is a conserved transmembrane protein that helps regulate cellular cholesterol levels through colocalization with NPC1. TMEM97 has also been linked to several cancers including breast, pancreatic, colorectal and ovarian cancers.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMEM97