Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]

Images

 
Western blot shows lysates of human kidney tissue, human heart tissue, and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Legumain/Asparaginyl Endopeptidase Antigen Affinity-purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Legumain/Asparaginyl Endopeptidase
Ile18-Tyr433
Accession # Q99538
Specificity
Detects human Legumain/Asparaginyl Endopeptidase in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LGMN
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunoprecipitation 25 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF2199 in the following applications:

Publications
Read Publications using
AF2199 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Legumain/Asparaginyl Endopeptidase Antibody [Unconjugated]

  • AEP
  • Asparaginyl Endopeptidase
  • cysteine protease 1
  • Legumain
  • LGMN
  • LGMN1
  • Protease, cysteine 1
  • protease, cysteine, 1 (legumain)
  • PRSC1EC 3.4.22.34

Background

Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin alpha to thymosin alpha 1 and thymosin alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by recombinant human (rh) Cystatin C and rhCystatin E/M and recombinant mouse Cystatin C (R&D Systems, Catalog # 1196‑PI, 1286-PI and 1238-PI, respectively).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Legumain/Asparaginyl Endopeptidase Antibody (AF2199)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Zebrafish.

We have publications tested in 3 applications: IHC - Paraffin embedded, IHC Fresh, WB.


Filter By Application
IHC - Paraffin embedded
(1)
IHC Fresh
(1)
WB
(5)
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Filter By Species
Human
(5)
Mouse
(1)
Zebrafish
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF2199 Applications Species
P Yao, Y Ding, Z Han, Y Mu, T Hong, Y Zhu, H Li Suppression of asparaginyl endopeptidase attenuates breast cancer-induced bone pain through inhibition of neurotrophin receptors Mol Pain, 2017;13(0):1.74481E+15. 2017 [PMID: 28554249] (WB,, Human) WB Human
The Agaricus blazei-Based Mushroom Extract, Andosan�, Protects against Intestinal Tumorigenesis in the A/J Min/+ Mouse PLoS ONE, 2016;11(12):e0167754. 2016 [PMID: 28002446] (IHC - Paraffin embedded,, Mouse) IHC - Paraffin embedded Mouse
Ma, Liping, Shen, Yan-Qin, Khatri, Harsh P, Schachner, Melitta The asparaginyl endopeptidase legumain is essential for functional recovery after spinal cord injury in adult zebrafish. PLoS ONE, 2014;9(4):e95098. 2014 [PMID: 24747977] (IHC Fresh,, Zebrafish) IHC Fresh Zebrafish
Smith R, Solberg R, Jacobsen L, Voreland A, Rustan A, Thoresen G, Johansen H Simvastatin inhibits glucose metabolism and legumain activity in human myotubes. PLoS ONE, 2014;9(1):e85721. 2014 [PMID: 24416446] (WB,, Human) WB Human
Differential secretome analysis reveals CST6 as a suppressor of breast cancer bone metastasis. Cell Res., 2012;22(9):1356-73. 2012 [PMID: 22688893] (WB,, Human) WB Human
Briggs JJ, Haugen MH, Johansen HT, Riker AI, Abrahamson M, Fodstad O, Maelandsmo GM, Solberg R Cystatin E/M suppresses legumain activity and invasion of human melanoma. BMC Cancer, 2010;10(1):17. 2010 [PMID: 20074384] (WB,, Human) WB Human
Clerin V, Shih HH, Deng N, Hebert G, Resmini C, Shields KM, Feldman JL, Winkler A, Albert L, Maganti V, Wong A, Paulsen JE, Keith JC, Vlasuk GP, Pittman DD Expression of the cysteine protease legumain in vascular lesions and functional implications in atherogenesis. Atherosclerosis, 2008;0(0):. 2008 [PMID: 18377911] (WB,, Human) WB Human

Reviews for Legumain/Asparaginyl Endopeptidase Antibody (AF2199) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF2199:
Filter by Applications
WB
(2)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot Legumain/Asparaginyl Endopeptidase AF2199
Enlarge
reviewed by:
Anonymous
WB Human 12/23/2015
View

Summary

ApplicationWestern Blot
Sample TestedHeLa and HEK293 whole cell lysate
SpeciesHuman
LotVCJ0115021

Blocking

Blocking Details30 minutes, 5%milk in TBST

Primary Anitbody

Dilution Ratioovernight, 4°C, 1:200 in 5%milk/TBST

Secondary Antibody

Secondary Descriptionanti-goat IgG, HRP
Secondary Concentration1:5000

Details

Detection Noteschemiluminiscence,exposure time 30 seconds
 
reviewed by:
Anonymous
WB Human 01/06/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22902879
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22902879

Details

Detection NotesSee PMID 22902879

Comments

CommentsThis antibody works very well both to western blotting and immuncytochemical staining.

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Secondary Antibodies

 

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Bioinformatics Tool for Legumain/Asparaginyl Endopeptidase Antibody (AF2199)

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Diseases for Legumain/Asparaginyl Endopeptidase Antibody (AF2199)

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Recent Reviews

4.5
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2
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Anonymous
12/23/2015
Application: WB
Species: Human

 
Anonymous
01/06/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol LGMN
Entrez
Uniprot