TMEM97 Antibody - BSA Free

Western Blot: TMEM97 Antibody [NBP1-30437] - Analysis of TMEM97 in NIH/3T3 using NBP1-30437.

• Reactivity: Mu
• Applications: WB, ICC/IF (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TMEM97 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TMEM97 Antibody - BSA Free (NBP1-30437) is a polyclonal antibody validated for use in WB. Anti-TMEM97 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of the mouse TMEM97 protein (within residues 20-100. [Swiss-Prot# Q8VD00]
• Localization: Membrane; Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TMEM97
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
• Application Notes: This TMEM97 antibody is useful for Western blot where a band is seen ~24 kDa. It is not recommended for ICC/IF.
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  TMEM97 Peptide (NBP1-30437PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TMEM97 Antibody - BSA Free

• MAC30
• MAC30Protein MAC30
• S2R
• Sigma2 receptor
• Sigma-2 receptor
• TMEM97
• transmembrane protein 97

Background

TMEM97 (transmembrane protein 97 or protein MAC30) is a member of IGFBP/insulin-like growth factor-binding protein family and is implicated in several biological processes wherein it acts through its regulatory effects on IGF activity. It is a multi-pass membrane protein which is localized in nuclear and cell membranes, RER and lysosomes, and is expressed in several normal tissues including brain, lung, heart, skeletal muscles, testis, ovary, and pregnant uterus. TMEM97 was originally identified as overexpressed in meningiomas, however, later it was found to exhibit altered expression in different types of cancers. The absence or downregulation of TMEM97 expression in pancreatic cancer suggests that it may acts as a tumor suppressor in pancreatic cancer. Contrastingly, its expression is stronger in breast, esophageal, stomach, colon, and oral squamous cell cancers than the corresponding normal tissues. Its physiological function is largely unknown, however, it has been suggested to implicate in cellular proliferation/differentiation and regulation of cholesterol homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM97 Antibody (NBP1-30437)(2)

Publications using NBP1-30437

• Peng M, Zhou X, Yao F et al. (-)-Epicatechin Provides Neuroprotection in Sodium Iodate-Induced Retinal Degeneration Frontiers in Medicine 2022-06-27 [PMID: 35833100] (Western Blot, Block/Neutralize)
• Fliesler SJ, Skelton LA, Rao SR TMEM97 localization in the adult mouse retina Investigative Ophthalmology & Visual Science 2022-01-01 (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: TMEM97
• Entrez:
  • 27346(Human)
  • 69071(Mouse)
• Uniprot:
  • Q5BJF2(Human)
  • Q8VD00(Mouse)