TMEM119 Antibody

Images

 
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Porcine cerebral cortex. 1:100 antibody dilution. 5 minute DAB stain. pH 6 Citrate buffer used for antigen retrieval by steam heat. Image from verified ...read more
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Staining of human cerebral cortex shows weak positivity in microglia.
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Staining of human lymph node shows moderate membranous positivity in germinal center cells.
Orthogonal Strategies: Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Staining in human lymph node and liver tissues. Corresponding TMEM119 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: TMEM119 Antibody [NBP2-30551] - Staining of human testis shows weak membranous positivity in cells in lamina propria.

Product Details

Summary
Reactivity Hu, Rt, Po, FeSpecies Glossary
Applications ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

TMEM119 Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: ITRQKQKASAYYPSSFPKKKYVDQSDRAGGPRAFSEVPDRAPDSRPEEALD
Specificity
Specificity of human, rat TMEM119 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TMEM119
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1 - 4 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID : 29109020). IHC-Frozen reactivity per customer review.
Control Peptide
TMEM119 Protein (NBP2-30551PEP)
Reviewed Applications
Read 2 Reviews rated 3.5
using
NBP2-30551 in the following applications:

Publications
Read Publication using
NBP2-30551 in the following applications:

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (86%), Rat reactivity reported in scientific literature (PMID: 29109020). Feline reactivity per customer review.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for TMEM119 Antibody

  • OBIF
  • Osteoblast Induction Factor
  • TMEM119

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TMEM119 Antibody (NBP2-30551)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(1)
All Applications
Filter By Species
Rat
(1)
All Species

Reviews for TMEM119 Antibody (NBP2-30551) (2) 3.52

Average Rating: 3.5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Feline, Swine.

Reviews using NBP2-30551:
Filter by Applications
IHC-P
(1)
IHC-Fr
(1)
All Applications
Filter by Species
Feline
(1)
Swine
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Paraffin TMEM119 NBP2-30551
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reviewed by:
Vicki Swier
IHC-P Swine 04/23/2018
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedBrain (cerebral cortex),Brain (hippocampus) tissue, brain (thalamus),brain (aqueduct)
SpeciesSwine
LotB114525

Comments

Comments1:100 dilution. 5 minute DAB stain. Polymer secondary. pH 6 Citrate buffer used for antigen retrieval by steam heat.
Immunohistochemistry-Frozen TMEM119 NBP2-30551
Enlarge
reviewed by:
Anonymous
IHC-Fr Feline 01/31/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedoptic nerve,Nerve pia mater
SpeciesFeline
LotB106244

Comments

CommentsPFA fixed and cryoprotected tissue.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for TMEM119 Antibody (NBP2-30551) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for TMEM119 Antibody (NBP2-30551)

Discover related pathways, diseases and genes to TMEM119 Antibody (NBP2-30551). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for TMEM119 Antibody (NBP2-30551)

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Pathways for TMEM119 Antibody (NBP2-30551)

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Blogs on TMEM119

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Recent Reviews

3.5
5
0
4
1
3
1
2
0
1
0

Vicki Swier
04/23/2018
Application: IHC-P
Species: Swine

Anonymous
01/31/2017
Application: IHC-Fr
Species: Feline

Bioinformatics

Gene Symbol TMEM119
Uniprot