![Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25724_rabbit-tmem119-mab-hl2415-azide-and-bsa-free-212024939115.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
| Reactivity | Mu, RtSpecies Glossary |
| Applications | IHC |
| Clone | HL2415 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant fragment of mouse TMEM119. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | TMEM119 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 29 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | No Preservative |
| Purity | Protein A purified |
![Immunohistochemistry PTH Antibody (918462) [Unconjugated]](https://images.novusbio.com/images2/PTH_MAB7665_Immunohistochemistry_16650.jpg)
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Secondary Antibodies |
Isotype Controls |
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Antibody treatment can generate microglia-like cells from bone marrow By Jennifer Sokolowski, MD, PhD.Microglia play important roles in the brain in both homeostatic and pathological conditions, acting to clear debris and dying cells. There is evidence to suggest that microglial dys... Read full blog post. |
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TMEM 119 is a specific marker of microglia cells By Jennifer Sokolowski, MD, PhD.Microglia are a major immune-cell component in the brain. They ingest and degrade dead cells, debris, and foreign material and interact with other immune cells to orchestrate centra... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | TMEM119 |
![Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25724_rabbit-tmem119-mab-hl2415-azide-and-bsa-free-212024940831.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:100. Red: beta Tubulin 3/ Tuj1 , a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25724_rabbit-tmem119-mab-hl2415-azide-and-bsa-free-2120249351314.jpg "Immunohistochemistry-Paraffin: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - TMEM119 antibody [HL2415] detects TMEM119 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. TMEM119 stained by TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (NBP2-43559) diluted at 1:500. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25724_rabbit-tmem119-mab-hl2415-azide-and-bsa-free-9102024201147.jpg "Western Blot: TMEM119 Antibody (HL2415) - Azide and BSA Free [NBP3-25724] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TMEM119 antibody [HL2415] (NBP3-25724) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
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![N/A SLPI [HRP]](https://images.novusbio.com/images2/DATA_SLPI_DPI00_ELISA_792.jpg)
![N/A SLPI [HRP]](https://images.novusbio.com/images2/SLPI_DPI00_ELISA_160.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")