TMEM119 Antibody (CL8714) - BSA Free

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - Analysis in human cerebral cortex and liver tissues. Corresponding TMEM119 RNA-seq data are presented for the same tissues.

Western Blot: TMEM119 Antibody (CL8714) [NBP2-76985] - Analysis in human cell line SH-SY5Y.

Immunocytochemistry/Immunofluorescence: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of SH-SY5Y cells using the Anti-TMEM119 monoclonal antibody, showing specific staining to the plasma membrane in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of human cerebellum shows strong membranous positivity in microglia.

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of human lymphoid tissues shows strong membranous positivity in germinal center cells.

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - TMEM119 on human FFPE tonsil, 1:100 and pH 9 antigen retrieval. Image submitted by a verified customer review.

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of human cerebral cortex shows strong membranous positivity in microglia.

Immunohistochemistry-Paraffin: TMEM119 Antibody (CL8714) [NBP2-76985] - Staining of human liver shows no positivity in hepatocytes as expected.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL8714
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

TMEM119 Antibody (CL8714) - BSA Free Summary

• Description: Novus Biologicals Mouse TMEM119 Antibody (CL8714) - BSA Free (NBP2-76985) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-TMEM119 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q4V9L6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMEM119
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/mL
  • Immunohistochemistry 1:5000 - 1:10000
  • Immunohistochemistry-Paraffin 1:5000 - 1:10000
  • Western Blot 1 ug/mL
• Application Notes: IHC, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for TMEM119 Antibody (CL8714) - BSA Free

• OBIF
• Osteoblast Induction Factor
• TMEM119

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TMEM119 Antibody (NBP2-76985)(1)

Publication using NBP2-76985

• DeMarino C, Lee MH, Cowen M et al. Detection of SARS-CoV-2 Nucleocapsid and Microvascular Disease in the Brain: A Case Report Neurology 2023-03-28 [PMID: 36526429] (Immunohistochemistry, Human)

Review for TMEM119 Antibody (NBP2-76985) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/12/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: human tonsil
• Species: Human
• Lot: I don't have it any more

Comments

• Comments: pH 9 antigen retrieval

Bioinformatics

• Gene Symbol: TMEM119