TMED1 Antibody (OTI7D2) - Azide and BSA Free

Western Blot: TMED1 Antibody (OTI7D2) - Azide and BSA Free [NBP2-74533] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TMED1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TMED1.

Immunocytochemistry/Immunofluorescence: TMED1 Antibody (OTI7D2) - Azide and BSA Free [NBP2-74533] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TMED1.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clone: OTI7D2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

TMED1 Antibody (OTI7D2) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TMED1 Antibody (OTI7D2) - Azide and BSA Free (NBP2-01277) is a monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human TMED1(NP_006849) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TMED1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500
• Theoretical MW: 22.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for TMED1 Antibody (OTI7D2) - Azide and BSA Free

• IL1RL1-Binding Protein
• Il1rl1l
• IL1RL1LG
• IL-1RL1LG
• IL1RL1LGIL1RL1-binding protein
• Interleukin 1 Receptor-Like 1 Ligand
• Interleukin-1 receptor-like 1 ligand
• Ly84l
• MGC1270
• P24g1
• P24gamma1
• Putative T1/ST2 receptor-binding protein
• ST2L
• T1/ST2 receptor binding protein
• TMED1
• Tp24
• transmembrane emp24 domain containing 1
• transmembrane emp24 domain-containing protein 1
• transmembrane emp24 protein transport domain containing 1

Background

The protein encoded by this gene was identified by its interaction with interleukin 1 receptor-like 1 (IL1RL1). This protein lacks any similarity to other interleukin 1 ligands. The functional significance of its interaction with IL1RL1 is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TMED1