TLE3 Antibody

Western blot shows lysates of MCF-7 human breast cancer cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse TLE3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5785) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TLE3 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

• Reactivity: Hu, Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TLE3 Antibody Summary

• Immunogen: E. coli-derived recombinant human TLE3
  His11-Asn200
  Accession # Q04726
• Specificity: Detects endogenous human TLE3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TLE3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLE3 Antibody

• ESG3
• ESG3Enhancer of split groucho-like protein 3
• ESGenhancer of split groucho 3
• Grg3
• HsT18976
• KIAA1547FLJ39460
• TLE3
• transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 3, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 3

Background

TLE3 (Transducin-Like Enhancer of Split 3; also ESG3 and GRG3) is an 84‑86 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed in the embryo, and in differentiating epithelial cells in the adult. TLE3 binds to a number of transcription factors as a heterooligomer, or to itself as a homotetramer. Oligomerization is essential for activity. Human TLE3is 772 amino acids (aa) in length. It contains a Gln(Q)-rich region that mediates oligomerization (aa 1‑131), a CCN domain that contains an NLS plus CKII and cdc2K kinase site (aa 199‑268), and seven WD repeats that mediate protein-protein interaction (aa 484‑771). Potentially there are multiple splice variants. Over aa 11‑200, human TLE3shares 99% aa identity wih mouse TLE3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLE3
• Uniprot:
  • Q04726()