TLE2 Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

TLE2 Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human TLE2
  Ile11-Ser193
  Accession # Q04725
• Specificity: Detects human TLE2 in direct ELISAs. In direct ELISAs, less than 2% cross-reactivity with recombinant human (rh) TLE1, rhTLE3, and rhTLE4 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TLE2 Antibody [Alexa Fluor® 488]

• enhancer of split groucho 2
• ESG
• ESG2
• ESG2Enhancer of split groucho-like protein 2
• FLJ41188
• GRG2
• TLE2
• transducin-like enhancer of split 2 (E(sp1) homolog, Drosophila)
• transducin-like enhancer of split 2, homolog of Drosophila E(sp1)
• transducin-like enhancer protein 2

Background

TLE2 (Transducin-Like Enhancer of Split 2; also ESG2 and Grg-2) is an 83-84 kDa member of the WD repeat Groucho/TLE family of transcriptional repressors. It is ubiquitously expressed, and is known to antagonize TCF (Wnt)-mediated signaling. TLE2 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of multiple factors such as FoxG1, Arx and histone H3. Human TLE2 is 743 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa 1-133), a CCN domain that contains an NLS (aa 195-256), and six WD repeats that mediate protein-protein interaction (aa 455-742). There are at least five potential phosphorylation sites that if used, may account for SDS-Page MWs exceeding 95 kDa. Potential isoform variants exist. One possesses a 21 aa substitution for aa 1-9, while a second shows the same substitution coupled to an additional 10 aa substitution for aa 683-743. A third isoform possesses an alternative start site at Met56 coupled to a deletion of aa 124-190. Over aa 11-193, human TLE2 shares 91% aa identity with mouse TLE2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.