Recombinant Human TL1A/TNFSF15 His Protein

Bioactivity: Recombinant Human TL1A/TNFSF15 His Protein [NBP1-72297] - Human TL1A/TNFSF15 is coated at 10 ug/ml (100 ul/well) can bind Human DR3/TNFRSF25 in a Functional ELISA assay.

SDS-Page: Recombinant Human TL1A/TNFSF15 His Protein [NBP1-72297] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Concentration: 0.5 mg/ml

Recombinant Human TL1A/TNFSF15 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 72-251 of Human TL1A/TNFSF15
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MLKGQEFAPS HQQVYAPLRA DGDKPRAHLT VVRQTPTQHF KNQFPALHWE HELGLAFTKN RMNYTNKFLL IPESGDYFIY SQVTFRGMTS ECSEIRQAGR PNKPDSITVV ITKVTDSYPE PTQLLMGTKS VCEVGSNWFQ PIYLGAMFSL QEGDKLMVNV SDISLVDYTK EDKTFFGAFL L

• Details of Functionality: Human TL1A/TNFSF15 when coated at 10 ug/ml (100 ul/well) can bind Human DR3/TNFRSF25 (NBP2-52352) in a Functional ELISA assay.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF15
• Purity: >90%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 22.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 40% glycerol, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human TL1A/TNFSF15 His Protein

• MGC129934
• MGC129935
• TL1A
• TL1Vascular endothelial cell growth inhibitor
• TNF superfamily ligand TL1A
• TNFSF15
• tumor necrosis factor (ligand) superfamily, member 15
• tumor necrosis factor ligand superfamily member 15
• vascular endothelial growth inhibitor-192A
• VEGI
• VEGI192A
• VEGITNF ligand-related molecule 1

Background

Tumor necrosis factor ligand superfamily member 15, also known TNFSF15, belongs to the TNF superfamily. TNFSF15 is predominantly expressed in endothelial cells and its expression is inducible by TNFalpha and IL1alpha. TNFSF15 is an angiogenesis inhibitor of the TNF family and functions in part by directly inhibiting endothelial cell proliferation. TNFSF15 may act as an autocrine factor to induce apoptosis in endothelial cells via activation of multiple signaling pathways, including stress protein kinases and certain caspases. Recombinant human TNFSF15 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF15