TIPRL Antibody

Western Blot: TIPRL Antibody [NBP1-51995] - (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TIPRL Antibody [NBP1-51995] - 3.75ug/ml staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TIPRL Antibody Summary

• Immunogen: Peptide with sequence C-RIDPNPADSQKSTQ corresponding to C-Terminus according to NP_690866.1.
• Epitope: C-RIDPNPADSQKSTQ
• Specificity: This antibody is expected to recognize isoform 1 (NP_690866.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TIPRL
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-3ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1:1000
• Application Notes: Western blot: Approx 30kDa band observed in lysates of cell line K562 (calculated MW of 31.4kDa according to NP_690866.1). Primary incubation was 1 hour
  IHC: Paraffin embedded Human Breast.

Reactivity Notes

Expected from sequence similarity: Mouse, Rat, Canine, Bovine

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TIPRL Antibody

• dJ69E11.3
• MGC3794
• putative MAPK activating protein
• Putative MAPK-activating protein PM10
• TIP41, TOR signaling pathway regulator-like (S. cerevisiae)
• TIP41-like protein
• TIP41Type 2A-interacting protein
• TIPTIP41, TOR signalling pathway regulator-like (S. cerevisiae)

Background

TIPRL is an inhibitory regulator of protein phosphatase-2A (PP2A) (see PPP2CA; MIM 176915), PP4 (see PPP4C; MIM 602035), and PP6 (see PPP6C; MIM 300141) (McConnell et al., 2007 [PubMed 17384681]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIPRL Antibody (NBP1-51995)(1)

Publication using NBP1-51995

• Ehret GB, O'Connor AA, Weder A et al. Follow-up of a major linkage peak on chromosome 1 reveals suggestive QTLs associated with essential hypertension: GenNet study. Eur J Hum Genet 2009-12-01 [PMID: 19536175]

Bioinformatics

• Gene Symbol: TIPRL
• Entrez:
  • 261726(Human)
  • 226591(Mouse)
  • 360869(Rat)
• Uniprot:
  • NP_690866.1(Human)