TIPIN Antibody (1G11) - Azide and BSA Free

Western Blot: TIPIN Antibody (1G11) [H00054962-M01] - Analysis of TIPIN expression in transfected 293T cell line by TIPIN monoclonal antibody (M01), clone 1G11.Lane 1: TIPIN transfected lysate(34.496 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: TIPIN Antibody (1G11) [H00054962-M01] - Analysis of monoclonal antibody to TIPIN on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: TIPIN Antibody (1G11) [H00054962-M01] - Detection limit for recombinant GST tagged TIPIN is 0.03 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 1G11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TIPIN Antibody (1G11) - Azide and BSA Free Summary

• Immunogen: TIPIN (AAH00870, 1 a.a. ~ 301 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLEPQENGVIDLPDYEHVEDETFPPFPPPASPERQDGEGTEPDEESGNGAPVPVPPKRTVKRNIPKLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIRLDLPILHEDFVSNNDEVAENNEHDVTSTELDPFLTNLSESEMFASELSRSLTEEQQQRIERNKQLALERRQAKLLSNSQTLGNDMLMNTPRAHTVEEVNTDEDQKEESNGLNEDILDNPCNDAIANTLNEEETLLDQSFKNVQQQLDATSRNITEAR
• Specificity: FLJ20516 - timeless-interacting protein
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIPIN
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA and immunofluorescence.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TIPIN Antibody (1G11) - Azide and BSA Free

• FLJ20516
• TIMELESS interacting protein
• TIMELESS-interacting protein

Background

Timeless-interacting protein (TIPIN) was originally isolated as a protein that interacts with timeless, a factor that plays a role in circadian rhythm and cell cycle checkpoint regulation. The TIM-TIPIN complex has been shown to coordinate the intra-S-phase checkpoint in response to UV-induced DNA damage. TIPIN is required for cell cycle arrest in response to DNA damage. It also interacts with RPA (replication protein A) on DNA and is able to slow down DNA chain elongation in response to UV-damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIPIN
• Entrez:
  • 54962(Human)
• Uniprot:
  • AAH00870(Human)