TIMM22 Antibody (JE66-67)

Western Blot: TIMM22 Antibody (JE66-67) [NBP3-33021] - Western blot analysis of TIMM22 on different lysates with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: MCF7 cell lysate (15 ug/Lane) Lane 5: HEK-293 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: Mouse testis tissue lysate (30 ug/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TIMM22 Antibody (JE66-67) [NBP3-33021] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: TIMM22 Antibody (JE66-67) [NBP3-33021] - Immunocytochemistry analysis of K-562 cells labeling TIMM22 with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: JE66-67
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TIMM22 Antibody (JE66-67) Summary

• Description: Novus Biologicals Rabbit TIMM22 Antibody (JE66-67) (NBP3-33021) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human TIMM22 aa 95-194 / 194. (Uniprot: Q9Y584)
• Localization: Mitochondrion inner membrane
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TIMM22
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:1000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TIMM22 Antibody (JE66-67)

• mitochondrial import inner membrane translocase subunit Tim22
• putative membrane protein
• Testis-expressed sequence 4TIM22TEX4
• translocase of inner mitochondrial membrane 22 homolog (yeast)

Background

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMM22