TIMM22 Antibodies
We offer TIMM22 Antibodies for use in common research applications: ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot. Each TIMM22 Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our TIMM22 Antibodies can be used in a variety of model species: Human. Use the list below to choose the TIMM22 Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our TIMM22 polyclonal antibodies.
Alternate Names for TIMM22 Antibodies
anti-TIMM22 antibody, anti-mitochondrial import inner membrane translocase subunit Tim22 antibody, anti-putative membrane protein antibody, anti-Testis-expressed sequence 4TIM22TEX4 antibody, anti-translocase of inner mitochondrial membrane 22 homolog (yeast) antibody
6 Results for "timm22" in Primary Antibodies
![Western Blot: TIMM22 Antibody (JE66-67) [NBP3-33021] - Western blot analysis of TIMM22 on different lysates with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: MCF7 cell lysate (15 ug/Lane) Lane 5: HEK-293 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: Mouse testis tissue lysate (30 ug/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33021_rabbit-timm22-mab-je66-67-28820241249349.jpg "Western Blot: TIMM22 Antibody (JE66-67) [NBP3-33021] - Western blot analysis of TIMM22 on different lysates with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/1,000 dilution. Lane 1: K-562 cell lysate (15 ug/Lane) Lane 2: HepG2 cell lysate (15 ug/Lane) Lane 3: A549 cell lysate (15 ug/Lane) Lane 4: MCF7 cell lysate (15 ug/Lane) Lane 5: HEK-293 cell lysate (15 ug/Lane) Lane 6: HeLa cell lysate (15 ug/Lane) Lane 7: Jurkat cell lysate (15 ug/Lane) Lane 8: Mouse testis tissue lysate (30 ug/Lane) Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33021) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: TIMM22 Antibody (JE66-67) [NBP3-33021] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33021) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33021_rabbit-timm22-mab-je66-67-29820241492677.jpg)
![Immunocytochemistry/ Immunofluorescence: TIMM22 Antibody (JE66-67) [NBP3-33021] - Immunocytochemistry analysis of K-562 cells labeling TIMM22 with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-TIMM22 antibody (NBP3-33021) at 1/100 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-33021_rabbit-timm22-mab-je66-67-29202410334098.jpg)
![Immunohistochemistry-Paraffin: TIMM22 Antibody [NBP1-92509] - Staining of human adrenal gland shows strong cytoplasmic in glandular cells.](http://images.novusbio.com/fullsize/TIMM22-Antibody-Immunohistochemistry-Paraffin-NBP1-92509-img0002.jpg "Immunohistochemistry-Paraffin: TIMM22 Antibody [NBP1-92509] - Staining of human adrenal gland shows strong cytoplasmic in glandular cells.")
![Western Blot: TIMM22 Antibody [NBP3-16088] - Western blot analysis of extracts of various cell lines, using TIMM22 antibody (NBP3-16088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/TIMM22-Antibody-Western-Blot-NBP3-16088-img0001.jpg "Western Blot: TIMM22 Antibody [NBP3-16088] - Western blot analysis of extracts of various cell lines, using TIMM22 antibody (NBP3-16088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunoprecipitation: TIMM22 Antibody [NBP3-16088] - Immunoprecipitation analysis of 600ug extracts of Rat brain cells using 3ug TIMM22 antibody (NBP3-16088). Western blot was performed from the immunoprecipitate using TIMM22 antibody (NBP3-16088) at a dilition of 1:500.](http://images.novusbio.com/fullsize/TIMM22-Antibody-Immunoprecipitation-NBP3-16088-img0002.jpg)
![Western Blot: TIMM22 Antibody (2H2R3) [NBP3-33438] - Western blot analysis of lysates from HepG2 cells, using TIMM22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33438_rabbit-timm22-mab-2h2r3-91220241421378.jpg "Western Blot: TIMM22 Antibody (2H2R3) [NBP3-33438] - Western blot analysis of lysates from HepG2 cells, using TIMM22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.")
![Western Blot: TIMM22 Antibody (2H2R3) [NBP3-33438] - Western blot analysis of various lysates, using TIMM22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-33438_rabbit-timm22-mab-2h2r3-912202414112884.jpg)
![Western Blot: TIMM22 Antibody (SR1314) [NBP3-22643] - Western blot analysis using NBP3-22643 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22643_rabbit-timm22-mab-sr1314-6720239533783.jpg "Western Blot: TIMM22 Antibody (SR1314) [NBP3-22643] - Western blot analysis using NBP3-22643 in A431 cell lysate.")
![Western Blot: TIMM22 Antibody [H00029928-B01P] - Analysis of TIMM22 expression in human pancreas.](http://images.novusbio.com/fullsize/TIMM22-Antibody-Western-Blot-H00029928-B01P-img0003.jpg "Western Blot: TIMM22 Antibody [H00029928-B01P] - Analysis of TIMM22 expression in human pancreas.")
![Western Blot: TIMM22 Antibody [H00029928-B01P] - Analysis of TIMM22 expression in transfected 293T cell line by TIMM22 polyclonal antibody. Lane 1: TIMM22 transfected lysate(21.34 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/TIMM22-Antibody-Western-Blot-H00029928-B01P-img0004.jpg)