TIMM22 Antibody (2H2R3)

Western Blot: TIMM22 Antibody (2H2R3) [NBP3-33438] - Western blot analysis of lysates from HepG2 cells, using TIMM22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

Western Blot: TIMM22 Antibody (2H2R3) [NBP3-33438] - Western blot analysis of various lysates, using TIMM22 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 2H2R3
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TIMM22 Antibody (2H2R3) Summary

• Description: Novus Biologicals Rabbit TIMM22 Antibody (2H2R3) (NBP3-33438) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 20-120aa of human TIMM22.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TIMM22
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA Recommended starting concentration is 1 ug/mL
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TIMM22 Antibody (2H2R3)

• mitochondrial import inner membrane translocase subunit Tim22
• putative membrane protein
• Testis-expressed sequence 4TIM22TEX4
• translocase of inner mitochondrial membrane 22 homolog (yeast)

Background

Essential core component of the TIM22 complex, a complex that mediates the import and insertion of multi-pass transmembrane proteins into the mitochondrial inner membrane. In the TIM22 complex, it constitutes the voltage-activated and signal-gated channel. Forms a twin-pore translocase that uses the membrane potential as external driving force in 2 voltage-dependent steps

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMM22