TIM-4 Antibody

Western Blot: TIM-4 Antibody [NBP1-03238] - TIM-4 in Jurkat lysate with TIM-4 antibody at (A) 1 and (B) 2 ug/ml.

Immunohistochemistry-Paraffin: TIM-4 Antibody [NBP1-03238] - Staining of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

TIM-4 Antibody Summary

• Immunogen: A 15 amino acid synthetic peptide near the C-terminus of human TIM-4. The immunogen is located within the last 50 amino acids of TIM-4.
• Epitope: C-Terminus
• Specificity: 15 amino acid peptide from near the carboxy terminus of human TIM-4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TIMD4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TIM-4 Antibody

• FLJ27515
• Smuckler
• T-cell immunoglobulin and mucin domain containing 4
• T-cell immunoglobulin and mucin domain-containing protein 4
• T-cell membrane protein 4
• TIM4
• TIM-4
• TIMD4
• TIMD-4

Background

The T cell immunoglobulin and mucin domain containing protein (TIM) family encodes cell surface receptors that are involved in the regulation of T helper (Th) -1 and -2 cell-mediated immunity. Studies have shown that TIM 4, which is preferentially expressed on macrophages and dendritic cells, is the natural ligand of TIM 1, and that this binding leads to T-cell expansion and cytokine production. Unlike other members of the TIM family, TIM 4 lacks a putative tyrosine phosphorylation signal sequence in its intracellular domain (Kuchroo et al, 2003). The TIM 4 gene maps to a locus associated with predisposition to asthma in both mice and humans and with its connection to TIM 1-triggered Th2 responsiveness, may be considered as a candidate disease/predisposition gene for asthma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TIMD4
• Entrez:
  • 91937(Human)
  • 276891(Mouse)
• Uniprot:
  • Q96H15()