TIM-4 Antibody (921832) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: Flow
• Clone: 921832
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

TIM-4 Antibody (921832) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NSO-derived recombinant human TIM-4
  Glu25-Leu315
  Accession # Q96H15
• Specificity: Detects human TIM-4 in direct ELISAs.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-4 Antibody (921832) [Alexa Fluor® 750]

• FLJ27515
• Smuckler
• T-cell immunoglobulin and mucin domain containing 4
• T-cell immunoglobulin and mucin domain-containing protein 4
• T-cell membrane protein 4
• TIM4
• TIM-4
• TIMD4
• TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The human TIM-4 cDNA encodes a 378 amino acid (aa) precursor that includes a 24 aa signal sequence, a 290 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1 or TIM-3. Within the ECD, human TIM-4 shares 35% and 23% aa sequence identity with TIM-1 and TIM-3, respectively. A TIM-2 ortholog has not been identified in human. The ECD of human TIM-4 shares 45% aa sequence identity with that of mouse and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for the hepatitis A virus, and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction of TIM-4 with TIM-1 induces costimulatory and hyperproliferative signals in T cells (5).

1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681. 
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q96H15()