TIM-4 Antibody (1018144) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with mouse TIM-4 and eGFP was stained with and either (A) Rat Anti-Mouse TIM-4 Monoclonal Antibody (Catalog # MAB2826) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by APC-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 1018144
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

TIM-4 Antibody (1018144) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line, NS0-derived mouse TIM-4
  Ala22-Thr279
  Accession # Q6U7R4
• Specificity: Detects mouse TIM-4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-4 Antibody (1018144) [Unconjugated]

• FLJ27515
• Smuckler
• T-cell immunoglobulin and mucin domain containing 4
• T-cell immunoglobulin and mucin domain-containing protein 4
• T-cell membrane protein 4
• TIM4
• TIM-4
• TIMD4
• TIMD-4

Background

TIM-4 (T cell; immunoglobulin; mucin-4), also known as SMUCKLER, is a 60 kDa member of the TIM family of immune regulating proteins. TIMs are type I transmembrane proteins with one Ig-like V domain and one Ser/Thr-rich mucin domain (1 - 3). The mouse TIM-4 cDNA encodes a 343 amino acid (aa) precursor that includes a 22 aa signal sequence, a 257 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 43 aa cytoplasmic tail (4). Structurally, TIM-4 is distinguished from other TIMs by the presence of an RGD motif in its Ig domain and the lack of a site for tyrosine phosphorylation in its cytoplasmic tail. The mucin domain in TIM-4 is larger than in TIM-1, -2, or -3. Within the ECD, mouse TIM-4 shares 27 - 33% aa sequence identity with mouse TIM-1, -2, and -3. The ECD of mouse TIM-4 shares 45% aa sequence identity with that of human and rat TIM-4. TIM-4 is expressed by macrophages and mature dendritic cells but not by lymphocytes (4, 5). TIM-4 binds specifically to TIM-1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma (5 - 7). Among hematopoietic cells, TIM-1 is expressed on activated B and T cells, preferentially in the Th2 subset of CD4⁺ T cells (5, 8). The interaction ofTIM-4 with TIM-1 induces co-stimulatory and hyperproliferative signals in T cells (5).

1. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362. 
4. Shakhov, A.N. et al. (2004) Eur. J. Immunol. 34:494. 
5. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
6. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
7. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q6U7R4()