TIM-3 Antibody (215008) [Phycoerythrin]

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TIM-3 PE-conjugated Monoclonal Antibody (Catalog # FAB1529P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 215008
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Phycoerythrin

TIM-3 Antibody (215008) [Phycoerythrin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIM‑3
  Arg20-Ala193
  Accession # Q8VIM0
• Specificity: Detects mouse TIM-3 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: HAVCR2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-3 Antibody (215008) [Phycoerythrin]

• CD366
• FLJ14428
• HAVCR2
• HAVcr-2
• hepatitis A virus cellular receptor 2
• kidney injury molecule-3
• KIM-3
• SPTCL
• T cell immunoglobulin mucin 3
• T cell immunoglobulin mucin-3
• T-cell immunoglobulin and mucin domain-containing protein 3
• TIM 3
• TIM3 T-cell membrane protein 3
• TIM3
• TIM-3
• TIMD3
• TIMD-3
• TIMD3KIM-3

Background

TIM-3 (T cell immunoglobulin and mucin domain-3) is a 60 kDa member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk (1 - 3). There are three TIM genes in human and eight in mouse. Mature mouse TIM-3 consists of a 174 aa extracellular domain (ECD), a 21 aa transmembrane segment (TM), and a 67 aa cytoplasmic tail (4). Two alternately spliced isoforms have been reported in mouse which lack either the TM or both the TM and mucin regions (5, 6). Within the ECD, mouse TIM-3 shares 58% and 74% aa sequence identity with human and rat TIM-3, respectively. TIM-3 is specifically expressed on Th1 cells whereas TIM-1 and TIM-2 are expressed on TH2 cells. In chronic inflammation, autoimmune disorders, and some cancers, TIM-3 is upregulated on several other hematopoietic cell types and on hippocampal neurons (9 - 12). The glycosylated Ig domain of TIM-3 binds cell-associated galectin-9 which induces TIM-3 Tyr phosphorylation and proapoptotic signaling(10, 13). TIM-3 functions as a negative regulator of Th1 cell activity. Its blockade results in increased IFN-gamma production, Th1 cell proliferation, and cytotoxicity (5, 7, 12, 14). TIM-3 may play a role in regulatory T cell development, (7) inflammation, (15) and immune tolerance (5, 13, 14). Soluble mouse TIM-3 has been shown to inhibit anti-tumor effector T cell responses and to enhance autoimmune reactions (6, 7).

1. Anderson, A.C. and D.E. Anderson (2006) Curr. Opin. Immunol. 18:665.
2. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.
3. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:362.
4. Monney, L. et al. (2002) Nature 415:536.
5. Sabatos, C.A. et al. (2003) Nat. Immunol. 4:1102.
6. Geng, H. et al. (2006) J. Immunol. 176:1411.
7. Sanchez-Fueyo, A. et al. (2003) Nat. Immunol. 4:1093.
8. Khademi, M. et al. (2004) J. Immunol. 172:7169.
9. Wiener, Z. et al. (2007) J. Invest. Dermatol. 127:906.
10. van de Weyer, P.S. et al. (2006) Biochem. Biophys. Res. Commun. 351:571.
11. Gielen, A.W. et al. (2005) J. Neuroimmunol. 164:93.
12. Oikawa, T. et al. (2006) J. Immunol. 177:4281.
13. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
14. Koguchi, K. et al. (2006) J. Exp. Med. 203:1413.
15. Frisancho-Kiss, S. et al. (2006) J. Immunol. 176:6411.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-3 Antibody (FAB1529P)(7)

Publications using FAB1529P

• M Hashimoto, K Araki, MA Cardenas, P Li, RR Jadhav, HT Kissick, WH Hudson, DJ McGuire, RC Obeng, A Wieland, J Lee, DT McManus, JL Ross, SJ Im, J Lee, JX Lin, B Hu, EE West, CD Scharer, GJ Freeman, AH Sharpe, SS Ramalingam, A Pellerin, V Teichgräbe, WJ Greenleaf, C Klein, JJ Goronzy, P Umaña, WJ Leonard, KA Smith, R Ahmed PD-1 combination therapy with IL-2 modifies CD8+ T cell exhaustion program Nature, 2022-09-28;610(7930):173-181. 2022-09-28 [PMID: 36171288] (Flow Cytometry, Mouse)
• CG Burke, JR Myers, CM Post, LA Boulé, BP Lawrence DNA Methylation Patterns in CD 4 + T Cells of Na�ve and Influenza A Virus-Infected Mice Developmentally Exposed to an Aryl Hydrocarbon Receptor Ligand Environmental health perspectives, 2021-01-15;129(1):17007. 2021-01-15 [PMID: 33449811] (Flow Cytometry, Mouse)
• MF Amani, AS Rolig, WL Redmond Intracellular Galectin-3 Is Essential for OX40-Mediated Memory CD8+ T Cell Development J. Immunol., 2020-08-26;0(0):. 2020-08-26 [PMID: 32848035] (Flow Cytometry, Mouse)
• IC Arnold, X Zhang, M Artola-Bor, A Fallegger, P Sander, P Johansen, A Müller BATF3-dependent dendritic cells drive both effector and regulatory T-cell responses in bacterially infected tissues PLoS Pathog., 2019-06-12;15(6):e1007866. 2019-06-12 [PMID: 31188899] (Flow Cytometry, Mouse)
• Cho J, Roche M, Sandall B, Brass A, Seed B, Xavier R, Medoff B Enhanced Tim3 activity improves survival after influenza infection. J Immunol, 2012-08-08;189(6):2879-89. 2012-08-08 [PMID: 22875804] (Flow Cytometry, Mouse)
• Reddy PB, Sehrawat S, Suryawanshi A Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency. J. Immunol., 2011-10-21;187(11):5745-55. 2011-10-21 [PMID: 22021615] (Flow Cytometry, Mouse)
• Sehrawat S, Suryawanshi A, Hirashima M, Rouse BT Role of Tim-3/galectin-9 inhibitory interaction in viral-induced immunopathology: shifting the balance toward regulators. J. Immunol., 2009-03-01;182(5):3191-201. 2009-03-01 [PMID: 19234217] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: HAVCR2
• Uniprot:
  • Q8VIM0()