TIM-2 Antibody [Unconjugated]

TIM‑2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse TIM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1885) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TIM-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2
  His22-Asn229
  Accession # NP_599010
• Specificity: Detects mouse TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human TIM-1 and recombinant mouse TIM-3 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Timd2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIM-2 Antibody [Unconjugated]

• G9D3
• T-cell immunoglobulin and mucin domain-containing protein 2
• T-cell immunoglobulin mucin receptor 2
• T-cell membrane protein 2
• TIM2
• TIM-2
• TIMD-2

Background

TIM-2 is a type I membrane protein and is a member of the TIM protein family. This protein family is characterized by their conserved immunoglobulin and mucin domains, as well as by their expression on activated T cells. TIM-2 is a receptor for Sema 4A, which is expressed on dendritic cells and B cells. Sema 4A has been shown to enhance T cell activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIM-2 Antibody (AF1885)(1)

Publication using AF1885

• Mogie G, Shanks K, Nkyimbeng-Takwi EH et al. Neuroimmune semaphorin 4A as a drug and drug target for asthma. Int Immunopharmacol 2013-08-28 [PMID: 23994348]

Bioinformatics

• Gene Symbol: Timd2
• Uniprot:
  • NP_599010()