TIGIT Antibody (2190A) [Unconjugated]

Mouse splenocytes were surface stained with Rat anti-Mouse CD4 FITC-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 2190A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

TIGIT Antibody (2190A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse TIGIT
  Met1-Thr143
  Accession # P86176
• Specificity: Detects mouse TIGIT in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIGIT Antibody (2190A) [Unconjugated]

• T cell immunoreceptor with Ig and ITIM domains
• TIGIT
• VSIG9
• VSTM3
• WUCAM

Background

TIGIT (T cell Immunoreceptor with Ig and ITIM domains; also Vstm3 and Vsig9) is a 30-34 kDa (in human) member of the CD28 family, Ig superfamily of molecules. It is expressed by NK cells and multiple subsets of mature T cells, and binds to PVR/CD155 and PVR2/CD112 that appear on dendritic cells (DC) and endothelium. Along with CD226, the TIGIT:CD226/DNAM1 and PVR:PVR2 pairings appear to form a network that parallels the well-characterized B7-1:B7-2 and CD28:CTLA4 system. Binding of TIGIT by DC induces DC IL-10 release and inhibits IL-12 production. Ligation of TIGIT on T cells dampens TCR-mediated activation, while NK cell TIGIT ligation blocks NK cell cytotoxicity. Mature mouse TIGIT is a type I transmembrane protein 215 amino acids (aa) in length. It contains a 116 aa extracellular region (aa 26-141) with a V-type Ig-like domain (aa 27-125), and a 79 aa cytoplasmic domain with one ITIM motif. There is one isoform variant that is quite unusual and shows an addition of nine amino acids spread over three insertion sites (SwissProt #:P86176). Mouse and human TIGIT are highly divergent, and over aa 26-143, mouse TIGIT shares only 68% aa sequence identity with human TIGIT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for TIGIT Antibody (MAB72671) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer Flow Mouse 11/16/2018

Summary

• Application: Flow Cytometry
• Sample Tested: 4T1 mouse breast cancer cell line
• Species: Mouse
• Lot: CLBM011809A

Comments

• Comments: Antibodies primary and secondary incubated for 60 min at 4 C . The antibody was tested along rabbit isotype control

reviewed by: Verified Customer Flow Mouse 10/24/2018

Summary

• Application: Flow Cytometry
• Sample Tested: MOuse cell line CT26
• Species: Mouse
• Lot: CLBM011809A

Bioinformatics

• Uniprot:
  • P86176(Mouse)