Nectin-2/CD112 Antibody [Unconjugated]

Images

 
Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed ...read more
Nectin‑2/CD112 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 1.7 µg/mL for 3 ...read more
Nectin‑2/CD112 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 3 µg/mL ...read more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line, ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Nectin-2/CD112 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Nectin‑2/CD112 isoform a
Gln32-Leu360
Accession # NP_002847
Specificity
Detects human Nectin‑2/CD112 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-3 and rhNectin-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PVRL2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2229 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-2/CD112 Antibody [Unconjugated]

  • CD112 antigen
  • CD112
  • Herpes virus entry mediator B
  • Herpesvirus entry mediator B
  • herpesvirus entry protein B
  • HVEB
  • HVEBpoliovirus receptor-like 2
  • MPH
  • nectin 2
  • Nectin2
  • Nectin-2
  • poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • poliovirus receptor-related protein 2
  • PRR2
  • PRR2nectin-2
  • PVRL2
  • PVRR2poliovirus receptor related 2

Background

Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types (2, 3). It has two splice forms (4, 5). Nectin-2δ is a 65 kDa long form and is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular region, a 21 aa TM segment, and a 157 aa cytoplasmic domain. The extracellular region contains one N-terminal 85 aa V-type Ig domain and two 45-55 aa C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (6). The short, 60 kDa isoform of Nectin-2 (Nectin-2 alpha ) has the same signal sequence and extracellular domain as nectin-2δ, but differs in the TM and cytoplasmic region (4, 5). In this case, the cytoplasmic tail is only 94 aa in length. The human extracellular region shows 72% aa sequence identity with the equivalent region in mouse. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.

  1. Takai, Y. and H. Nakanishi, 2003, J. Cell Sci. 116:17.
  2. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  3. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  4. Eberle, F. et al. (1995) Gene 159:267.
  5. Warner, M.S. et al. (1998) Virology 246:179.
  6. Struyf, F. et al. (2002) J. Virol. 76:12940.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB25301
Species: Hu
Applications: CyTOF-ready, Flow, ICC, WB
MAB666
Species: Hu
Applications: CyTOF-ready, Flow, Neut, WB
AF2880
Species: Hu, Mu
Applications: WB
MAB356
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), Flow, WB
MAB139
Species: Hu
Applications: CostimT, CyTOF-ready, Flow, Neut, WB
NB110-60531
Species: Hu, Mu, Rt(-)
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, WB
AF3064
Species: Hu
Applications: CyTOF-ready, Flow, IHC, WB
MAB224
Species: Hu
Applications: CyTOF-ready, Flow, IHC, Neut
AF6695
Species: Mu, Rt
Applications: CyTOF-ready, Flow, IHC, WB
AF7829
Species: Hu
Applications: ICC, IHC, WB
7268-CT
Species: Hu
Applications: BA
NB300-186
Species: Hu, Rt
Applications: ICC/IF, Simple Western, WB
AF1730
Species: Hu
Applications: AdBlk, CyTOF-ready, Flow, ICC
NB100-524
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
NBP2-79843
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
1849-NK
Species: Hu
Applications: BA
NB500-328
Species: Hu
Applications: CyTOF-ready, Flow, IP
AF2225
Species: Mu
Applications: AgAct, CyTOF-ready, Flow, IHC, WB

Publications for Nectin-2/CD112 Antibody (AF2229)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
IHC
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF2229 Applications Species
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022;13(1):1606. 2022 [PMID: 35338135] (Western Blot, Human) Western Blot Human
Y Li, Y Zhang, G Cao, X Zheng, C Sun, H Wei, Z Tian, W Xiao, R Sun, H Sun Blockade of checkpoint receptor PVRIG unleashes anti-tumor immunity of NK cells in murine and human solid tumors Journal of hematology & oncology, 2021;14(1):100. 2021 [PMID: 34174928] (IHC, Human) IHC Human
R Molfetta, ND Milito, B Zitti, M Lecce, C Fionda, M Cippitelli, A Santoni, R Paolini The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30888046] (Immunoprecipitation, Human) Immunoprecipitation Human
Q Zhang, J Bi, X Zheng, Y Chen, H Wang, W Wu, Z Wang, Q Wu, H Peng, H Wei, R Sun, Z Tian Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity Nat. Immunol., 2018;0(0):. 2018 [PMID: 29915296] (IHC-P, Human) IHC-P Human
C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma Oncotarget, 2017;8(4):5954-5964. 2017 [PMID: 27992381] (Western Blot, Human) Western Blot Human
Herron CR, Lowery AM, Hollister PR, Reynolds AB, Vincent PA p120 regulates endothelial permeability independently of its NH2 terminus and Rho binding. Am. J. Physiol. Heart Circ. Physiol., 2011;300(1):H36-48. 2011 [PMID: 20971762] (Western Blot, Human) Western Blot Human

Reviews for Nectin-2/CD112 Antibody (AF2229) (0)

There are no reviews for Nectin-2/CD112 Antibody (AF2229). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Nectin-2/CD112 Antibody (AF2229) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Nectin-2/CD112 Products

Bioinformatics Tool for Nectin-2/CD112 Antibody (AF2229)

Discover related pathways, diseases and genes to Nectin-2/CD112 Antibody (AF2229). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Nectin-2/CD112 Antibody (AF2229)

Discover more about diseases related to Nectin-2/CD112 Antibody (AF2229).
 

Pathways for Nectin-2/CD112 Antibody (AF2229)

View related products by pathway.

Blogs on Nectin-2/CD112

There are no specific blogs for Nectin-2/CD112, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Nectin-2/CD112 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PVRL2
Uniprot