Nectin-2/CD112 Antibody [Unconjugated]


Western blot shows lysates of 293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line. PVDF membrane was probed more
Nectin‑2/CD112 was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 1.7 µg/mL for 3 more
Nectin‑2/CD112 was detected in immersion fixed paraffin-embedded sections of human liver using Goat Anti-Human Nectin‑2/CD112 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2229) at 3 µg/mL more
Simple Western lane view shows lysates of HEK293T human embryonic kidney cell line, A549 human lung carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and Raji human Burkitt's lymphoma cell line, more

Product Details

Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF

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Nectin-2/CD112 Antibody [Unconjugated] Summary

Mouse myeloma cell line NS0-derived recombinant human Nectin‑2/CD112 isoform a
Accession # NP_002847
Detects human Nectin‑2/CD112 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) Nectin-1, rhNectin-3 and rhNectin-4 is observed.
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 3-15 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF2229 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nectin-2/CD112 Antibody [Unconjugated]

  • CD112 antigen
  • CD112
  • Herpes virus entry mediator B
  • Herpesvirus entry mediator B
  • herpesvirus entry protein B
  • HVEB
  • HVEBpoliovirus receptor-like 2
  • MPH
  • nectin 2
  • Nectin2
  • Nectin-2
  • poliovirus receptor-related 2 (herpesvirus entry mediator B)
  • poliovirus receptor-related protein 2
  • PRR2
  • PRR2nectin-2
  • PVRL2
  • PVRR2poliovirus receptor related 2


Nectins are a small family of Ca++-independent immunoglobulin (Ig)-like cell adhesion molecules (CAMs) that organize intercellular junctions (1). The nectin family has at least four members (nectin-1-4), all of which show alternate splicing (except for Nectin-4), a transmembrane (TM) region (except for Nectin-1 gamma ), and three extracellular Ig-domains. Nectins are highly homologous to the human receptor for poliovirus, and as such have been alternately named poliovirus receptor-related proteins. They do not, however, appear to bind poliovirus (1). Nectin-2 is a 60 or 65 kDa type I TM glycoprotein that is found on a variety of cell types (2, 3). It has two splice forms (4, 5). Nectin-2δ is a 65 kDa long form and is synthesized as a 538 amino acid precursor. It contains a 31 amino acid (aa) signal sequence, a 329 aa extracellular region, a 21 aa TM segment, and a 157 aa cytoplasmic domain. The extracellular region contains one N-terminal 85 aa V-type Ig domain and two 45-55 aa C2-type Ig domains. The V-domain is believed to mediate nectin binding to its ligands (6). The short, 60 kDa isoform of Nectin-2 (Nectin-2 alpha ) has the same signal sequence and extracellular domain as nectin-2δ, but differs in the TM and cytoplasmic region (4, 5). In this case, the cytoplasmic tail is only 94 aa in length. The human extracellular region shows 72% aa sequence identity with the equivalent region in mouse. Nectin-2 is known to bind the pseudorabies virus, and herpes simplex virus-2 (HSV-2), but not HSV-1. It does not bind poliovirus. As a cell adhesion molecule, Nectin-2 will form cis-homodimers (same cell), followed by trans-dimers (across cells). Nectin-2 will not cis-dimerize with other nectins, but will cis-dimerize with its two splice forms. Notably, a Nectin-2 cis-dimer on one cell will heterodimerize with a Nectin-3 cis-dimer on another cell (1). Nectin-2 is found concentrated in adherens junctions, and exists on neurons, endothelial cells, epithelial cells and fibroblasts.

  1. Takai, Y. and H. Nakanishi, 2003, J. Cell Sci. 116:17.
  2. Bottino, C. et al. (2003) J. Exp. Med. 198:557.
  3. Pende, D. et al. (2005) Mol. Immunol. 42:463.
  4. Eberle, F. et al. (1995) Gene 159:267.
  5. Warner, M.S. et al. (1998) Virology 246:179.
  6. Struyf, F. et al. (2002) J. Virol. 76:12940.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nectin-2/CD112 Antibody (AF2229)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: IHC, IHC-P, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 6 of 6.
Publications using AF2229 Applications Species
J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity Nature Communications, 2022;13(1):1606. 2022 [PMID: 35338135] (Western Blot, Human) Western Blot Human
Y Li, Y Zhang, G Cao, X Zheng, C Sun, H Wei, Z Tian, W Xiao, R Sun, H Sun Blockade of checkpoint receptor PVRIG unleashes anti-tumor immunity of NK cells in murine and human solid tumors Journal of hematology & oncology, 2021;14(1):100. 2021 [PMID: 34174928] (IHC, Human) IHC Human
R Molfetta, ND Milito, B Zitti, M Lecce, C Fionda, M Cippitelli, A Santoni, R Paolini The Ubiquitin-proteasome pathway regulates Nectin2/CD112 expression and impairs NK cell recognition and killing Eur. J. Immunol., 2019;0(0):. 2019 [PMID: 30888046] (Immunoprecipitation, Human) Immunoprecipitation Human
Q Zhang, J Bi, X Zheng, Y Chen, H Wang, W Wu, Z Wang, Q Wu, H Peng, H Wei, R Sun, Z Tian Blockade of the checkpoint receptor TIGIT prevents NK cell exhaustion and elicits potent anti-tumor immunity Nat. Immunol., 2018;0(0):. 2018 [PMID: 29915296] (IHC-P, Human) IHC-P Human
C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma Oncotarget, 2017;8(4):5954-5964. 2017 [PMID: 27992381] (Western Blot, Human) Western Blot Human
Herron CR, Lowery AM, Hollister PR, Reynolds AB, Vincent PA p120 regulates endothelial permeability independently of its NH2 terminus and Rho binding. Am. J. Physiol. Heart Circ. Physiol., 2011;300(1):H36-48. 2011 [PMID: 20971762] (Western Blot, Human) Western Blot Human

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Gene Symbol PVRL2