FDC Antibody (CNA.42)

Images

 
Immunohistochemistry-Paraffin: FDC Antibody (CNA.42) [NBP1-43118] - Formalin-fixed paraffin embedded human tonsil using 1 ug/mL Mouse IgM Isotype Control (left) or 1 ug/mL Anti-Human FDC Purified (right) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, IHC-Fr, IHC-P
Clone
CNA.42
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.5 mg/ml

FDC Antibody (CNA.42) Summary

Immunogen
The immunogen for this antibody was follicular dendritic cells.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Gene
CMD1B
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This CNA.42 antibody has been tested by flow cytometric of human cell line and western blotting. This can be used at less than or equal to 0.5 ug per million cells in a 100 ul total staining volume or 2ug/ml (for western blotting).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Aqueous, may contain carrier protein/stabilizer.
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for FDC Antibody (CNA.42)

  • cardiomyopathy, dilated 1B (autosomal dominant)
  • CMPD1
  • FDC

Background

The monoclonal antibody CNA.42 recognizes a unique carbohydrate epitope found on follicular dendritic cells (FDCs) from a variety of species. FDCs are large cells in germinal centers (GCs) of primary and lymphoid tissues. They present non-processed antigen and switch off apoptosis of B cells through a selection process resulting in selection of memory B cells. FDCs express many B cell markers such as CD21 (long isoform), CD23 and CD35. A role for FDCs as been shown in various infectious diseases such as AIDS and transmissible spongiform encephalitis. Staining with this CNA.42 monoclonal antibody is mainly restricted to the plasma membrane but in limited cases may also be cytoplasmic (as seen in mast cells). This monoclonal has been shown to be a useful reagent for both flow cytometry and IHC analysis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-43118AF405
Alexa Fluor 488 Labeled NBP1-43118AF488
Alexa Fluor 647 Labeled NBP1-43118AF647
Alexa Fluor 700 Labeled NBP1-43118AF700
Allophycocyanin Labeled NBP1-43118APC
DyLight 350 Labeled NBP1-43118UV
DyLight 405 Labeled NBP1-43118V
DyLight 488 Labeled NBP1-43118G
DyLight 680 Labeled NBP1-43118FR
DyLight 755 Labeled NBP1-43118IR
PE Labeled NBP1-43118PE
PerCP Labeled NBP1-43118PCP

Additional FDC Products

FDC NBP1-43118

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Bioinformatics

Gene Symbol CMD1B