TIGIT Antibodies

FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P)." class="big_lightbox"> FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P)." alt="Mouse splenocytes were surface stained with Rat anti-Mouse CD4 FITC-conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rabbit Anti-Mouse TIGIT Monoclonal Antibody (Catalog # MAB72671) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Then stained with Rabbit anti-Human/Mouse FoxP3 PE-conjugated Monoclonal Antibody (Catalog # IC8214P)." class="big_thumb" />

F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_lightbox"> F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." alt="Human peripheral blood lymphocytes were stained with Mouse Anti-Human TIGIT Monoclonal Antibody (Catalog # MAB7898) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on control antibody staining (Catalog # MAB0041)." class="big_thumb" />

F0127) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." class="big_lightbox"> F0127) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." alt="Mouse splenocytes treated with 50 ng/mL PMA and 500 ng/mL Calcium ionomycin for 24 hours were stained with Sheep Anti-Mouse TIGIT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7267) followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127) and Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P). Quadrant markers were set based on control antibody staining (Catalog # 5-001-A)." class="big_thumb" />

VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with either (A) cynomolgus monkey TIGIT or (B) irrelevant protein, and eGFP, was stained with Rabbit anti-cynomolgus monkey TIGIT monoclonal antibody (Catalog # MAB10532) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on control antibody staining (MAB1050, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

9380-TG) binds to immobilized Recombinant Human CD155/PVR (2530-CD) coated at 2.5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Cynomolgus Monkey TIGIT Monoclonal Antibody (Catalog # MAB10532). At 70-350 ng/mL, this antibody will block 50% of the binding." class="big_lightbox"> 9380-TG) binds to immobilized Recombinant Human CD155/PVR (2530-CD) coated at 2.5 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Cynomolgus Monkey TIGIT Monoclonal Antibody (Catalog # MAB10532). At 70-350 ng/mL, this antibody will block 50% of the binding." class="big_thumb" />