Tight Junction Protein 1 Antibody - BSA Free

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining in human cerebral cortex and skeletal muscle tissues using anti-TJP1 antibody. Corresponding TJP1 RNA-seq data are presented for the same tissues.

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Analysis of Tight Junction Protein 1 in RIPA whole cell lysates of mouse primary choroid plexus epithelial cells using anti-Tight Junction Protein 1 antibody. Image from verified customer review.

Immunocytochemistry/Immunofluorescence: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cell line U-2 OS shows localization to cytosol & cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human cerebral cortex shows high expression.

Immunohistochemistry-Paraffin: Tight Junction Protein 1 Antibody [NBP1-85046] - Staining of human skeletal muscle shows low expression as expected.

Western Blot: Tight Junction Protein 1 Antibody [NBP1-85046] - Expression of tight junction proteins in cerebellum of rats with mild liver damage. Expression of (A,B) occludin & (C,D) ZO-1 at 2 & 4 weeks was analyzed by Western blot. Values are mean ± SEM of 10–12 rats per group. One-way ANOVA with Tukey’s test was performed for occludin at 2 weeks (F(3,27) = 4.99, p 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p 0.05) & for ZO-1 at 2 weeks (W(3,23) = 0.715, p > 0.05) & 4 weeks (F(3,43) = 3.698, p < 0.05). Values significantly different from control rats are indicated by asterisks & from CCl4 rats by a. * p < 0.05; a p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34440206), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Tight Junction Protein 1 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: ASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGR
• Marker: Intercellular Junctions/Tight Junction Marker
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TJP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25 - 2 ug/mL
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Frozen validated from a verified customer review.
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot validated from a verified customer review.
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control:

  Human Brain Cerebral Meninges Membrane Tissue Lysate (Adult Membrane Normal)

• Control Peptide:

  Tight Junction Protein 1 Protein (NBP1-85046PEP)

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 31174067).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Tight Junction Protein 1 Antibody - BSA Free

• DKFZp686M05161
• MGC133289
• tight junction protein 1 (zona occludens 1)
• Tight junction protein 1
• tight junction protein ZO-1
• TJP1
• ZO1
• ZO-1
• zona occludens 1
• Zona occludens protein 1
• zonula occludens 1 protein
• Zonula occludens protein 1

Background

Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-1, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes. ZO-1 is a peripheral membrane protein bound on the cytoplasmic surface of junctional contacts and is expressed in all tight junctions regardless of their properties. ZO-1 immunoprecipitates with its family member ZO-2. ZO-1 was shown to undergo tyrosine phosphorylation during tight junction formation and remodeling. Two different isoforms of ZO-1, alpha-minus and alpha-plus, have been described, which result from alternative splicing of an mRNA encoded by a single gene. The ZO-1 alpha-plus contains an 80 amino acids motif called alpha which is not present in ZO-1 alpha-minus. The alpha-containing isoform is found in most epithelial cell junctions. The short isoform (ZO-1 alpha-minus) is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. This difference in distribution provides molecular distinction among tight junctions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tight Junction Protein 1 Antibody (NBP1-85046)(15)

Publications using NBP1-85046

• Han B, Lv X, Liu G et al. Gut microbiota-related bile acid metabolism-FXR/TGR5 axis impacts the response to anti-?4?7-integrin therapy in humanized mice with colitis Gut microbes 2023-07-11 [PMID: 37431863] (WB)
• Mona K, Marnick C, Sumin C et al. Anatomical barriers against SARS-CoV-2 neuroinvasion at vulnerable interfaces visualized in deceased COVID-19 patients Neuron 2022-11-01 [PMID: 36446381] (IF/IHC, Human)
• Shastri S, Shinde T, Woolley KL et al. Short-Chain Naphthoquinone Protects Against Both Acute and Spontaneous Chronic Murine Colitis by Alleviating Inflammatory Responses Frontiers in Pharmacology 2021-08-23 [PMID: 34497514]
• Balzano T, Leone P, Ivaylova G Et al. Rifaximin Prevents T-Lymphocytes and Macrophages Infiltration in Cerebellum and Restores Motor Incoordination in Rats with Mild Liver Damage Biomedicines 2021-08-12 [PMID: 34440206] (WB, Rat)
• Lensu S, Pariyani R, MAkinen E et al. Prebiotic Xylo-oligosaccharides Targeting Faecalibacterium prausnitzii Prevent High Fat Diet-induced Hepatic Steatosis in Rats Nutrients 2020-10-27 [PMID: 33105554]
• Patterson L, Allen J, Posey I et al. Glucosylceramide production maintains colon integrity in response to Bacteroides fragilis toxin-induced colon epithelial cell signaling FASEB J 2020-10-13 [PMID: 33047400]
• Ko SF, Chen KH, Wallace CG et al. Protective effect of combined therapy with hyperbaric oxygen and autologous adipose-derived mesenchymal stem cells on renal function in rodent after acute ischemia-reperfusion injury Am J Transl Res 2020-07-15 [PMID: 32774699] (IF/IHC, Mouse)
• Shi Y, Li R, Yang J et al. No tight junctions in tight junction protein-1 expressing HeLa and fibroblast cells Int J Physiol Pathophysiol Pharmacol 2020-05-19 [PMID: 32067099] (IF/IHC, Mouse)
• Sheu JJ, Sung PH, Wallace CG et al. Intravenous administration of iPS-MSCSPIONs mobilized into CKD parenchyma and effectively preserved residual renal function in CKD rat J. Cell. Mol. Med. 2020-02-15 [PMID: 32061051] (IF/IHC, Rat)
• Shastri S, Shinde T, Sohal SS et al. Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms Int J Mol Sci 2020-01-12 [PMID: 31940911] (IF/IHC, Mouse)
• Evran S, Calis F, Akkaya E et al. The effect of high mobility group box-1 protein on cerebral edema, blood-brain barrier, oxidative stress and apoptosis in an experimental traumatic brain injury model Brain Res. Bull. 2019-11-09 [PMID: 31715313] (IF/IHC, Rat)
• Yip HK, Chen KH, Dubey NK, Sun CK et al. Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model Biomaterials 2019-05-28 [PMID: 31174067] (IHC-P, Rat)
• Shinde, T;Perera, AP;Vemuri, R;Gondalia, SV;Karpe, AV;Beale, DJ;Shastri, S;Southam, B;Eri, R;Stanley, R; Synbiotic Supplementation Containing Whole Plant Sugar Cane Fibre and Probiotic Spores Potentiates Protective Synergistic Effects in Mouse Model of IBD Nutrients 2019-04-11 [PMID: 30979002] (IF/IHC, Mouse)
• Gonzalez-Andrades M, Garzon I, Gascon MI et al. Sequential development of intercellular junctions in bioengineered human corneas. J Tissue Eng Regen Med 2009-08-01 [PMID: 19415764]
• Gangl K, Reininger R, Bernhard D et al. Cigarette smoke facilitates allergen penetration across respiratory epithelium. Allergy 2009-03-01 [PMID: 19120070]

Reviews for Tight Junction Protein 1 Antibody (NBP1-85046) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Mouse, Rat.

reviewed by: Verified Customer IHC-Fr Rat 05/21/2021

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Rat intestine
• Species: Rat
• Lot: B107603

reviewed by: Verified Customer WB Mouse 08/26/2015

Summary

• Application: Western Blot
• Sample Tested: RIPA whole cell lysates of mouse primary choroid plexus epithelial cells
• Species: Mouse

Bioinformatics

• Gene Symbol: TJP1