Claudin-5 Antibody (OTI1G4)

Images

 
Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody (OTI1G4) [NBP2-02259] - Claudin-5 labeled pig SC cells visualized by alexa 594. ICC/IF image submitted by a verified customer review.
Flow Cytometry: Claudin-5 Antibody (1G4) [NBP2-02259] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Claudin 5 antibody, and then ...read more
Immunocytochemistry/ Immunofluorescence: Claudin-5 Antibody (1G4) [NBP2-02259] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Claudin 5.

Product Details

Summary
Reactivity Hu, Mu, Rt, PoSpecies Glossary
Applications Flow, ICC/IF
Clone
OTI1G4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Claudin-5 Antibody (OTI1G4) Summary

Immunogen
Full length human recombinant protein of human CLDN5(NP_003268) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CLDN5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
Application Notes
Claudin-5 antibody validated for ICC/IF from a verified customer review.
Theoretical MW
23 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-02259 in the following applications:

Reactivity Notes

Porcine reactiviy reported from a verifed customer review. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Claudin-5 Antibody (OTI1G4)

  • AWAL
  • AWALTransmembrane protein deleted in VCFS
  • BEC1
  • claudin 5
  • Claudin5
  • Claudin-5
  • CLDN5
  • CPETRL1
  • TMDVCF
  • TMVCF1
  • TMVCFTMDVCF
  • transmembrane protein deleted in velocardiofacial syndrome

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: Flow, ICC/IF

Publications for Claudin-5 Antibody (NBP2-02259) (0)

There are no publications for Claudin-5 Antibody (NBP2-02259).
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Review for Claudin-5 Antibody (NBP2-02259) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.

Reviews using NBP2-02259:
Filter by Applications
Immunocytochemistry
(1)
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Pig
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Images Ratings Applications Species Date Details
Immunocytochemistry Claudin-5 NBP2-02259
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5
reviewed by:
L P
Immunocytochemistry Pig 06/07/2017
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Summary

ApplicationImmunocytochemistry
Sample Testedpig SC cells
SpeciesPig
LotN/A

Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Claudin-5 Antibody (NBP2-02259) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
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3
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2
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1
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L P
06/07/2017
Application: Immunocytochemistry
Species: Pig

Bioinformatics

Gene Symbol CLDN5
Entrez