Recombinant Human TIGAR/C12orf5 TAT Protein

SDS-Page: Human TIGAR/C12orf5 TAT Protein [NBP2-35063]

• Reactivity: Hu
• Applications: PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human TIGAR/C12orf5 TAT Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 283 amino acids corresponding to TIGAR/C12orf5 Source: E. coli

  Uniprot ID: Q9NQ88

  Amino Acid Sequence: MARFALTVVR HGETRFNKEK IIQGQGVDEP LSETGFKQAA AAGIFLNNVK FTHAFSSDLM RTKQTMHGIL ERSKFCKDMT VKYDSRLRER KYGVVEGKAL SELRAMAKAA REECPVFTPP GGETLDQVKM RGIDFFEFLC QLILKEADQK EQFSQGSPSN CLETSLAEIF PLGKNHSSKV NSDSGIPGLA ASVLVVSHGA YMRSLFDYFL TDLKCSLPAT LSRSELMSVT PNTGMSLFII NFEEGREVKP TVQCICMNLQ DHLNGLTETR GGYGRKKRRQ RRR

• Details of Functionality: TIGAR protein is fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 ug/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TIGAR
• Purity: >95%, by SDS-PAGE and HPLC
• Endotoxin Note: Less than 0.1 EU/ug of TIGAR/C12orf5 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 31.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 30 % Acetonitrile, 0.1% TFA.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >95%, by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human TIGAR/C12orf5 TAT Protein

• C12orf5
• chromosome 12 open reading frame 5
• EC 3.1.3.46
• probable fructose-2,6-bisphosphatase TIGAR
• TIGAR
• TIGARTP53-induced glycolysis and apoptosis regulator
• transactivated by NS3TP2 protein

Background

The TP53-inducible glycolysis and apoptosis regulator (TIGAR), also named fructose-2, 6-bisphosphatase TIGAR, is an enzyme that in humans is encoded by the C12orf5 gene. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. TIGAR activity can have multiple cellular effects. Recombinant human TIGAR-TAT expressed in E. coli is a 31.7 kDa protein containing 283 amino-acid residues, including the 270 residues of fulllength TIGAR fused to a 13-residue C-terminal peptide containing the TAT transduction domain (GGYGRKKRRQRRR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TIGAR
• Uniprot:
  • Q9NQ88(Human)