TIGAR/C12orf5 Overexpression Lysate (Native)

Images

 
Western Blot: TIGAR Overexpression Lysate (Adult Normal) [NBL1-08147] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIGAR.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TIGAR/C12orf5 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204087 and based on accession number NM_020375. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens chromosome 12 open reading frame 5 (C12orf5), mRNA.
Gene
C12ORF5

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
29.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TIGAR/C12orf5

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TIGAR/C12orf5 Overexpression Lysate (Native)

  • C12orf5
  • chromosome 12 open reading frame 5
  • EC 3.1.3.46
  • probable fructose-2,6-bisphosphatase TIGAR
  • TIGAR
  • TIGARTP53-induced glycolysis and apoptosis regulator
  • transactivated by NS3TP2 protein

Background

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TIGAR/C12orf5 Lysate (NBL1-08147) (0)

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Bioinformatics

Gene Symbol C12ORF5
Entrez