Thyroid Peroxidase Antibody

Images

 
Western Blot: Thyroid Peroxidase Antibody [NBP2-27544] - (0.1 ug/ml) Analysis of Thyroid Gland lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP2-27544] - Staining of Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Thyroid Peroxidase Antibody Summary

Immunogen
Peptide with sequence ELRCGKHQAVGTSPQ corresponding to C-Terminus according to NP_000538.3, NP_783650.1, NP_783652.1, NP_783653.1.
Specificity
This antibody is expected to recognize all reported isoforms (NP_000538.3; NP_783650.1; NP_783652.1; NP_783653.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TPO
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 110 kDa band observed in human thyroid gland lysates (calculated MW of 103 kDa band according to NP_000538.3). IHC-P: Human thyroid gland shows textured staining in the cytoplasm of epithelial cells.
Publications
Read Publications using
NBP2-27544 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Thyroid Peroxidase Antibody

  • EC 1.11.1
  • MSA
  • TDH2A
  • thyroid microsomal antigen
  • thyroid peroxidase
  • thyroperoxidase
  • TPXEC 1.11.1.8

Background

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thyroid Peroxidase Antibody (NBP2-27544)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TPO
Uniprot