Thyroid Peroxidase Antibody

Western Blot: Thyroid Peroxidase Antibody [NBP1-97765] - (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-97765] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Thyroid Peroxidase Antibody Summary

• Immunogen: Peptide with sequence C-TRHVIQVSNEVVTDD corresponding to internal region according to NP_000538.3, NP_783650.1, NP_783652.1, NP_783653.1.
• Specificity: This antibody is expected to recognize reported isoforms a (NP_000538.3) and e (NP_783653.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 110 kDa band observed in human thyroid gland lysates (calculated MW of 103 kDa band according to NP_000538.3). IHC-P: Human thyroid gland shows textured staining in the cytoplasm of epithelial cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Thyroid Peroxidase Antibody

• EC 1.11.1
• MSA
• TDH2A
• thyroid microsomal antigen
• thyroid peroxidase
• thyroperoxidase
• TPXEC 1.11.1.8

Background

Thyroid Peroxidase encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Additional splice variants have been described but their biological natures have not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thyroid Peroxidase Antibody (NBP1-97765)(1)

Publication using NBP1-97765

• Chen CR, Hamidi S, Braley-Mullen H et al. Antibodies to thyroid peroxidase arise spontaneously with age in NOD.H-2h4 mice and appear after thyroglobulin antibodies. Endocrinology 2010-09-01 [PMID: 20573721]

Bioinformatics

• Gene Symbol: TPO
• Entrez:
  • 7173(Human)
• Uniprot:
  • NP_000538.3(Human)