Thyroid Peroxidase Antibody

Images

 
Western Blot: Thyroid Peroxidase Antibody [NBP1-97765] - (0.3ug/ml) staining of Human Thyroid Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Thyroid Peroxidase Antibody [NBP1-97765] - Staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Thyroid Peroxidase Antibody Summary

Immunogen
Peptide with sequence C-TRHVIQVSNEVVTDD corresponding to internal region according to NP_000538.3, NP_783650.1, NP_783652.1, NP_783653.1.
Specificity
This antibody is expected to recognize reported isoforms a (NP_000538.3) and e (NP_783653.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TPO
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 110 kDa band observed in human thyroid gland lysates (calculated MW of 103 kDa band according to NP_000538.3). IHC-P: Human thyroid gland shows textured staining in the cytoplasm of epithelial cells.
Publications
Read Publication using NBP1-97765.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Thyroid Peroxidase Antibody

  • EC 1.11.1
  • MSA
  • TDH2A
  • thyroid microsomal antigen
  • thyroid peroxidase
  • thyroperoxidase
  • TPXEC 1.11.1.8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Thyroid Peroxidase Antibody (NBP1-97765)(1)

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Bioinformatics

Gene Symbol TPO
Entrez
Uniprot