Thymidylate Synthase Antibody - BSA Free

Immunohistochemistry;Thymidylate Synthase Antibody[NBP3-29829]Detection of human TS by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma and lung cancer . Antibody: Affinity purified rabbit anti-TS used at a dilution of 1:1,000 . Detection: DAB

Immunohistochemistry;Thymidylate Synthase Antibody[NBP3-29828]Detection of mouse TS by immunohistochemistry. Samples: FFPE sections of mouse teratoma and plasmacytoma . Antibody: Affinity purified rabbit anti-TS used at a dilution of 1:1,000 . Detection: DAB

Immunoprecipitation:Thymidylate Synthase AntibodyNBP3-29829] -Detection of human TS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-TS antibody NBP3-29829 (lot NBP3-29829-1) used for IP at 6 µg/mg lysate. TS was also immunoprecipitated by rabbit anti-TS antibody for blotting immunoprecipitated TS, NBP3-29829 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Thymidylate Synthase Antibody - BSA Free Summary

• Immunogen: Between 263 and 313
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TYMS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for Thymidylate Synthase Antibody - BSA Free

• EC 2.1.1.45
• HsT422
• MGC88736
• OK/SW-cl.29
• Thymidylate Synthase
• thymidylate synthetase
• TMSTsase
• TS
• TSase
• TSHST422
• TYMS

Background

Thymidylate synthase (TS) is an intracellular enzyme critical for de novo synthesis of DNA. In cancer, expression of TS is often elevated and becomes further elevated as a result of treatment with the most commonly used chemotherapeutic, 5-fluorouracil (5-FU). Resistance or lack of response to 5-FU is attributed to the elevation of TS activity. Overexpression of TS has been demonstrated in many types of tumors including breast, colon, gastric and melanoma and is usually predictive of shortened survival time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYMS