Thymidine Kinase 2 Antibody - BSA Free

Immunohistochemistry: Thymidine Kinase 2 Antibody - BSA Free [NBP3-47480] - Immunohistochemistry of paraffin-embedded rat kidney tissue slide using (Thymidine Kinase 2 Antibody) at dilution of 1:100

Western Blot: Thymidine Kinase 2 Antibody - BSA Free [NBP3-47480] - Mouse liver were subjected to SDS PAGE followed by western blot with (Thymidine Kinase 2 antibody) at dilution of 1:1000

• Reactivity: Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Thymidine Kinase 2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Thymidine Kinase 2 Antibody - BSA Free (NBP3-47480) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: thymidine kinase 2, mitochondrial. (Uniprot# O00142)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:50-1:500
  • Western Blot 1:200-1:2000
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Thymidine Kinase 2 Antibody - BSA Free

• EC 2.7.1.21
• MTDPS2
• MTTK
• mt-TK
• thymidine kinase 2, mitochondrial

Background

The mitochondrial deoxyribonucleotide (dNTP) pool is separated from the cytosolic pool because the mitochondria inner membrane is impermeable to charged molecules. The mitochondrial pool is maintained by either import of cytosolic dNTPs through dedicated transporters or by salvaging deoxynucleosides within the mitochondria; apparently, enzymes of the de novo dNTP synthesis pathway are not present in the mitochondria. In nonreplicating cells, where cytosolic dNTP synthesis is downregulated, mtDNA synthesis depends solely on the mitochondrial salvage pathway enzymes, the deoxyribonucleoside kinases. Two of the 4 human deoxyribonucleoside kinases, deoxyguanosine kinase (DGK) and thymidine kinase-2, are expressed in mitochondria. Human DGK, encoded by the DGUOK gene (MIM 601465), efficiently phosphorylates deoxyguanosine and deoxyadenosine, whereas TK2 phosphorylates deoxythymidine, deoxycytidine, and deoxyuridine. Thymidine kinase-2 (TK2) is a deoxyribonucleoside kinase that phosphorylates thymidine, deoxycytidine, and deoxyuridine, and also phosphorylates antiviral and anticancer nucleoside analogs.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TK2