Thrombomodulin/BDCA-3 Antibody (501733) [Unconjugated]

Images

 
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human HLA‑DR APC‑conjugated Monoclonal Antibody (Catalog # FAB4869A) and either (A) Mouse Anti-Human Thrombomodulin/BDCA‑3 ...read more
Thrombomodulin/BDCA-3 was detected in immersion fixed paraffin-embedded sections of human kidney using 25 µg/mL Mouse Anti-Human Thrombomodulin/BDCA-3 Monoclonal Antibody (Catalog # MAB3947) overnight at 4 °C. Tissue ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready, ELISA(Cap)
Clone
501733
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Thrombomodulin/BDCA-3 Antibody (501733) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Thrombomodulin/BDCA-3
Ala19-Ser515
Accession # P07204(Val473)
Specificity
Detects human Thrombomodulin/BDCA-3 in ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
THBD
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
Application Notes
ELISA Capture: Human Thrombomodulin/BDCA-3 Antibody (Catalog # MAB3947)
ELISA Detection: Human Thrombomodulin/BDCA-3 Biotinylated Antibody (Catalog # BAM39471)
Standard: Recombinant Human Thrombomodulin/BDCA-3 (Catalog # 3947-PA)
Publications
Read Publications using
MAB3947 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thrombomodulin/BDCA-3 Antibody (501733) [Unconjugated]

  • BDCA3
  • BDCA-3
  • CD141 antigen
  • CD141
  • Fetomodulin
  • THBD
  • THRM
  • Thrombomodulin
  • TM

Background

Encoded by the THBD gene, Thrombomodulin is also known as blood dendritic cell antigen 3 (BDCA‑3) and designated CD141. The deduced amino acid (aa) sequence of human THBD predicts a signal peptide (aa 1‑18) and a mature chain (aa 19‑575) that consists of following domains: C‑type lectin (aa 31‑169), EGF‑like (aa 241‑281, aa 284‑324, aa 325‑363, aa 365‑405, aa 404‑440, and aa 441‑481), transmembrane (aa 516 to 539) and cytoplasmic (aa 540‑575). The region used as an immunogen consists of aa 19‑515, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis (1‑3). It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin‑activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C‑terminal aa residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (4). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis (5).

  1. Van de Wouwer, M. et al. (2004) Arterioscler. Thromb. Vasc. 24:1374.
  2. Wu, K.K. et al. (2000) Ann. Med. 32:73.
  3. Li, Y.H. et al. (2006) Cardiovasc. Hematol. Agents Med. Chem. 4:183.
  4. Weiler, H. and B.H. Isermann (2003) J. Thromb. Haemost. 1:1515.
  5. Califano, F. et al. (2000) Eur. Rev. Med. Pharmacol. Sci. 4:59.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thrombomodulin/BDCA-3 Antibody (MAB3947)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: ELISA Development (Capture), Flow Cytometry, ICC.


Filter By Application
ELISA Development (Capture)
(1)
Flow Cytometry
(1)
ICC
(1)
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Human
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Secondary Antibodies

 

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Additional Thrombomodulin/BDCA-3 Products

Blogs on Thrombomodulin/BDCA-3.

Thrombomodulin - A multifunctional protein with roles in inflammation and coagulation
Thrombomodulin, also known as BDCA-3, is a glycosylated transmembrane protein present on the surface of vascular endothelial cells. Thrombomodulin is a high-affinity receptor for thrombin, a key protein in the coagulation cascade. Formation of the...  Read full blog post.

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Bioinformatics

Gene Symbol THBD
Uniprot