Thrombomodulin/BDCA-3 Antibody (4D8.2C6) - BSA Free

Immunohistochemistry-Paraffin: Thrombomodulin/BDCA-3 Antibody (4D8.2C6) [NBP2-36496] - Immunohistochemistry on mouse kidney using 10 ug/ml of Thrombomodulin antibody (NBP2-36496)

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clone: 4D8.2C6
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Thrombomodulin/BDCA-3 Antibody (4D8.2C6) - BSA Free Summary

• Immunogen: Two synthetic peptides made to internal portions of the mouse Thrombomodulin protein (between residues 25-150 and 400-550) [UniProt P07204]
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Gene: THBD
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2 ug / 1 million cells
  • Immunohistochemistry-Paraffin
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Thrombomodulin/BDCA-3 Antibody (4D8.2C6) - BSA Free

• BDCA3
• BDCA-3
• CD141 antigen
• CD141
• Fetomodulin
• THBD
• THRM
• Thrombomodulin
• TM

Background

Encoded by the THBD gene, Thrombomodulin is also known as CD141 antigen. The deduced amino acid sequence of mouse THBD predicts a signal peptide (aa 1 to 16) and a mature chain (aa 17 to 577) that consists of the following domains: C-type lectin (aa 31 to 167), EGF-like (aa 240 to 280, aa 283 to 323, aa 324 to 362, aa 364 to 404, aa 405 to 439, and aa 440 to 480), transmembrane (aa 518 to 541) and cytoplasmic (aa 542 to 577) (1). The R&D Systems rmTHBD consists of aa 17 to 517, corresponding to the extracellular portion of the type I membrane protein. Predominantly synthesized by vascular endothelial cells, THBD inhibits coagulation and fibrinolysis. It functions as a cell surface receptor and an essential cofactor for active thrombin, which in turn activates protein C and thrombin-activatable fibrinolysis inhibitor (TAFI), also known as carboxypeptidase B2 (CPB2). Activated protein C (APC), facilitated by protein S, degrades coagulation factors Va and VIIIa, which are required for thrombin activation. Activated CPB2 cleaves basic C-terminal amino acid residues of its substrates, including fibrin, preventing the conversion of plasminogen to plasmin. In addition, THBD gene polymorphisims are associated with human disease and THBD plays a role in thrombosis, stroke, arteriosclerosis, and cancer (5). For example, increased serum levels of THBD, due to protease cleavage, have been associated with smoking, cardiac surgery, atherosclerosis, liver cirrhosis, diabetes mellitus, cerebral and myocardial infarction, and multiple sclerosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THBD
• Entrez:
  • 21824(Mouse)