Carboxypeptidase B2/CPB2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human platelets. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Carboxypeptidase B2/CPB2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6036) followed by ...read more
Carboxypeptidase B2/CPB2 was detected in immersion fixed paraffin-embedded sections of normal human liver (left panel) and human liver cancer tissue (right panel) using Sheep Anti-Human Carboxypeptidase B2/CPB2 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Carboxypeptidase B2/CPB2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase B2/CPB2
Phe23-Val423 (Ala169Thr)
Accession # Q96IY4
Specificity
Detects Carboxypeptidase B2/CPB2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CPA1, rhCPB1 and recombinant mouse CPB1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
CPB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase B2/CPB2 Antibody [Unconjugated]

  • carboxypeptidase B2 (plasma)
  • carboxypeptidase B2 (plasma, carboxypeptidase U)
  • Carboxypeptidase B2
  • Carboxypeptidase U
  • CPB2
  • CPU
  • CPUEC 3.4.17.20
  • EC 3.4.17
  • PCPB
  • Plasma carboxypeptidase B
  • TAFI
  • TAFIcarboxypeptidase B-like protein
  • Thrombin-activable fibrinolysis inhibitor
  • thrombin-activatable fibrinolysis inhibitor

Background

CPB2 (Carboxypeptidase B2; also CPU and TAFI) is a secreted, 50-60 kDa glycoprotein member of the peptidase M14 family of enzymes. It is expressed by hepatocytes (60 kDa) and platelets (50 kDa), with MW differences attributable to glycosylation. CPB2 is cleaved by thrombin and plasmin, generating a 36 kDa, relatively insoluble nonglycosylated enzymatically active fragment (TAFIa). Active CPB2 removes C-terminal Lys residues from fibrin, thereby interrupting plasmin generation and promoting fibrin polymerization. Human CPB2 (proprecursor/zymogen) is 401 amino acids (aa) in length. It contains a prosequence (aa 23-114) and an active fragment (aa 115-423) that acts on C-terminal Lys or Arg residues. There is one potential isoform variant that shows a deletion of aa 198-234 accompanied by a 16 aa substitution for aa 382-423. Over aa 23-423, human CPB2 shares 85% aa identity with mouse CPB2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol CPB2
Uniprot