Thimet Oligopeptidase/THOP1 Antibody [Biotin]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Biotin
• Concentration: LYOPH

Thimet Oligopeptidase/THOP1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human Thimet Oligopeptidase/THOP1
  Lys2-Cys689
  Accession # P52888
• Specificity: Detects human Thimet Oligopeptidase/THOP1 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human Neurolysin is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: THOP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Thimet Oligopeptidase/THOP1 Antibody [Biotin]

• EC 3.4.24
• EC 3.4.24.15
• Endopeptidase 24.15
• EP24.15
• MEPD_HUMAN
• MP78
• thimet oligopeptidase 1
• thimet oligopeptidase
• THOP1
• TOP

Background

Thimet Oligopeptidase/THOP1, also known as endopeptidase EC 3.4.24.15 (EP24.15), is a zinc peptidase of the M3 family that also includes neurolysin/EC 3.4.24.16 and mitochondrial intermediate peptidase (1). Widely expressed by mammalian tissues and reported to present in different subcellular locations, THOP1 is primarily a cytoplasmic enzyme. It is capable of hydrolyzing a number of bioactive peptides and peptides released by the proteosome, limiting antigenic presentation by MHC class I molecules (1‑3). THOP1 also interacts with angiotensin II type I receptor and bradykinin B2 receptor (4). The optimal activity of the purified THOP1 may or may not require the presence of a reducing agent, depending upon the source of the enzyme and the purification method used.

1. Barrett, A.J. and J.-M. Chen (2004) in Handbook of Proteolytic Enzymes. Barrett, A.J. et al. eds. p. 352, Elsevier Academic Press, San Diego.
2. Ray, K. et al. (2004) J. Biol. Chem. 279:20480.
3. Saric, T. et al. (2004) J. Biol. Chem. 279:46723.
4. Shivakumar, B.R. et al. (2005) Cell Biochem. Funct. 23:195.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Thimet Oligopeptidase/THOP1 Antibody (BAF3439)(2)

Publications using BAF3439

• Yanaika S. Hok‐A‐Hin, Katharina Bolsewig, Daimy N. Ruiters, Alberto Lleó, Daniel Alcolea, Afina W. Lemstra et al. Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease: A cross‐platform validation study Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring 2023-07-26 [PMID: 37502019]
• Yanaika S. Hok‐A‐Hin, Katharina Bolsewig, Daimy N. Ruiters, Alberto Lleó, Daniel Alcolea, Afina W. Lemstra et al. Thimet oligopeptidase as a potential CSF biomarker for Alzheimer's disease: A cross‐platform validation study Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring 2023-07-26 [PMID: 37502019] (Simple Plex Automated Immunoassay, Human)

Bioinformatics

• Gene Symbol: THOP1
• Uniprot:
  • P52888()