Thimet Oligopeptidase/THOP1 Antibody (4D6) [DyLight 405]


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Product Details

Reactivity Hu, Mu, Rt, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
DyLight 405

Thimet Oligopeptidase/THOP1 Antibody (4D6) [DyLight 405] Summary

A synthetic peptide corresponding to residues 56-72 of human Thimet Oligopeptidase. [UniProt# P52888]
Protein A purified
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  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This Thimet Oligopeptidase (4D6) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~80 kDa. For IHC-P, this antibody has been tested in human, monkey, and rat brain and testis. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, mouse, rat, rabbit, and monkey.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein A purified


Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Thimet Oligopeptidase/THOP1 Antibody (4D6) [DyLight 405]

  • EC 3.4.24
  • EC
  • Endopeptidase 24.15
  • EP24.15
  • MP78
  • thimet oligopeptidase 1
  • thimet oligopeptidase
  • THOP1
  • TOP


Thimet oligopeptidase (THOP1) is a thiol-rich enzyme with a HEXXH catalytic motif containing zinc distributed ubiquitously in mammalian cells and it belongs to family of M3 metallopeptidases. THOP1 is an oligopeptidase, as it only degrades short peptides (5-17 amino acids) and because of thiol compounds mediated activation, it is referred to as thimet. THOP1 implicates in degradation of peptides released by the 26 S proteasome and is prone to oxidative oligomerization through formation of interprotein disulfides involving specific Cys residues. Maximal THOP1 activity is maintained by partial S-glutathionylation, which is a mechanism that regulates protein oligomerization and enzymatic activity. Besides exising as a secreted neuropeptide-metabolizing enzyme, it is predominately expressed in cytoplasm and nucleus of neuronal cells. THOP1 is involved in cytoplasmic peptide degradation and cleaves bioactive peptides inside and outside CNS (including degradation of beta-amyloid precursor protein and generation of amyloidogenic fragments). It has also been shown to hydrolyses bradykinin (BK), neurotensin (NT), GnRH (gonadotrophin-release hormone), beta-neoendorphin, alpha-neoendorphin, dynorphin 1-8 and nociceptin/orphanin in vitro. THOP1 activity may be controlled by phosphorylation and/or by intermolecular disulfide bonds leading to inactive oligomers, and it expression is differentially regulated in neuroendocrine and spermatid cell lineages by a transcription factor binding to SRY (sex-determining region Y), CAAT box and CREB (cAMP-response element-binding protein) promoter consensus sequences.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

Other Available Formats

Biotin Labeled NB400-146B
DyLight 350 Labeled NB400-146UV
DyLight 488 Labeled NB400-146G
DyLight 550 Labeled NB400-146R
DyLight 650 Labeled NB400-146C
DyLight 680 Labeled NB400-146FR
DyLight 755 Labeled NB400-146IR
FITC Labeled NB400-146F
HRP Labeled NB400-146H

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Gene Symbol THOP1