THAP11 Antibody (605383) [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for THAP11 at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

THAP11 was detected in immersion fixed undifferentiated BG01V human embryonic stem cells using Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.2 mg/mL. A specific band was detected for THAP11 at approximately 56 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse THAP11 Monoclonal Antibody (Catalog # MAB5727). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF
• Clone: 605383
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

THAP11 Antibody (605383) [Unconjugated] Summary

• Immunogen: E. coli-derived THAP11
  Gly226-Met314
  Accession # Q96EK4
• Specificity: Detects human and mouse THAP11 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: THAP11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Simple Western 20 ug/mL
  • Western Blot 2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for THAP11 Antibody (605383) [Unconjugated]

• CTG-B43a
• CTG-B45d
• HRIHFB2206
• Ronin
• THAP domain containing 11
• THAP domain-containing protein 11
• THAP11

Background

THAP11, also known as Ronin, is a 36-44 kDa member of the thanatos-associated protein family. It is ubiquitously expressed and through its repression of c-Myc transcription, blocks cell proliferation. Human THAP11 is 314 amino acids (aa) in length. It contains a DNA-binding THAP zinc-finger domain (aa 5-81), an NLS (aa 98-104), a polyGln region (aa 104-146), an Ala-rich segment (aa 197-221) and a C-terminal coiled-coil region (aa 255-305). THAP11 is phosphorylated on Thr25. Over aa 226-314, human and mouse THAP11 are 100% identical in amino acid sequence.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for THAP11 Antibody (MAB5727)(4)

Publications using MAB5727

• Melissa L. Hancock, Rebecca C. Meyer, Meeta Mistry, Radhika S. Khetani, Alexandre Wagschal, Taehwan Shin et al. Insulin Receptor Associates with Promoters Genome-wide and Regulates Gene Expression Cell 2019-04-01 [PMID: 30955890]
• Vinckevicius A, Parker JB, Chakravarti D. Genomic Determinants of THAP11/ZNF143/HCFC1 Complex Recruitment to Chromatin. Mol Cell Biol. 2015-12-15 [PMID: 26416877]
• J. Brandon Parker, Hanwei Yin, Aurimas Vinckevicius, Debabrata Chakravarti Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143 Cell Reports 2014-11-06 [PMID: 25437553]
• J. Brandon Parker, Hanwei Yin, Aurimas Vinckevicius, Debabrata Chakravarti Host Cell Factor-1 Recruitment to E2F-Bound and Cell-Cycle-Control Genes Is Mediated by THAP11 and ZNF143 Cell Reports 2014-11-06 [PMID: 25437553] (Immunoprecipitation (ChIP), Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: THAP11
• Uniprot:
  • Q96EK4()