TH1L Antibody

Western Blot: TH1L Antibody [NBP1-52112] - (0.5ug/ml) staining of nuclear Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TH1L Antibody Summary

• Description: Novus Biologicals Goat TH1L Antibody (NBP1-52112) is a polyclonal antibody validated for use in WB and ELISA. Anti-TH1L Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NKRVSINKDE corresponding to internal region according to NP_945327.1.
• Epitope: C-NKRVSINKDE
• Predicted Species: Mouse (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NELFCD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5 - 2 ug/ml
• Application Notes: WB: Approx. 60 kDa band observed in nuclear Jurkat lysates (calculated MW of 66.2 kDa band according to NP_945327.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TH1L Antibody

• HSPC130
• negative elongation factor proteins C and D
• NELF-C
• NELF-C/D
• NELFD
• NELF-D
• TH1 drosophila homolog
• TH1-like (Drosophila homolog)
• TH1-like (Drosophila)
• TH1-like protein
• TH1negative elongation factor C/D
• trihydrophobin 1

Background

The NELF complex of proteins interacts with the DSIF protein complex to repress transcriptional elongation by RNA polymerase II. The protein encoded by this gene is an essential part of the NELF complex. Alternative translation initiation site usage results in the formation of two isoforms with different N-termini.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TH1L Antibody (NBP1-52112)(1)

Publication using NBP1-52112

• Yang Y, Zou W, Kong X et al. Trihydrophobin 1 attenuates androgen signal transduction through promoting androgen receptor degradation. J Cell Biochem 2010-04-01 [PMID: 20069563]

Bioinformatics

• Gene Symbol: NELFCD
• Entrez:
  • 51497(Human)
  • 57314(Mouse)
• Uniprot:
  • NP_945327.1(Human)