TGIF1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

TGIF1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human TGIF1
  Lys245-Ser359
  Accession # Q15583
• Specificity: Detects human TGIF1 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TGIF1 Antibody [Alexa Fluor® 594]

• 5'-TG-3' interacting factor
• homeobox protein TGIF
• homeobox protein TGIF1
• HPE4
• MGC39747
• TALE homeobox TG-interacting factor
• TGFB-induced factor (TALE family homeobox)
• TGFB-induced factor homeobox 1
• TGIF1
• TGIFMGC5066,5'-TG-3'-interacting factor 1
• transforming growth factor-beta-induced factor

Background

TGIF1 (5'-TG[TCA]-3' Interacting Protein 1; also Transforming growth factor beta-induced factor and HPE4) is a 43 kDa (predicted) member of the TALE/TGIF homeobox family of proteins. It is widely expressed, and serves as a transcriptional corepressor for SMAD2-induced gene activation. Evidence suggests that it either complexes with c-jun and cPML, or interacts with HDAC in the nucleus to perform this function. It also binds to RXR response elements, blocking RXR-mediated gene activation. Such interactions are of considerable importance during fetal and neonatal development. Human TGIF1 is 401 amino acids (aa) in length. It contains a DNA-binding homeobox domain (aa 164-226) and two consecutive repressor domains (aa 267-401). There are multiple splice variants. One is very well characterized, is 33-35 kDa in size, and shows a four aa substitution for aa 1-133. This is most active when phosphorylated, which adds approximately 2-4 kDa to its SDS-PAGE MW. Two other splice forms contain a 19 aa substitution for aa 1-134, and utilize an alternative start site at Met150, respectively. Over aa 245-359, human TGIF1 shares 81% aa identity with mouse TGIF1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.